Author：C. Y. Chiang, H. J. Chang, M. C. Chuang, T. H. Huang, Y. J. Chao, L. J. Fong, S. Tejakhod, C. Y. Chu*

Department of Horticulture, National Chung Hsing University, Taiwan
* Author for correspondence: chychu@dragon.nchu.edu.tw

Abstract：

    Plant tissue culture is an efficient technique when it comes to plant propagation. The feasibility of combining plant tissue culture with mutagenesis through chemical or physical agent has been tested in our lab. Crops tested include chrysanthemum, Dieffenbachia, Gerbera, poinsettia and Kalanchoe. The higher mutation rates of most chrysanthemums were obtained from callus nuder γ-ray irradiation at 4 Gy. As well as Dieffenbachia was obtained at 1 Gy. The mutation types from compositae flower bud explants had more varieties than those obtained from callus explants, and that replacing γ-ray with NaN₃ as mutagen caused severe decrease in mutagenesis efficiency. The poinsettia hypocotyl explants cut from mature embryos were used and exposed under γ-ray or were subcultured on regenerating medium containing NaN₃. In the end, we found that physical mutagen treatment was more suitable for organgensis and chemical mutagen was more suitable for embryogenesis. lower buds of kalanchoe were exposed to NaN₃, and mutation rates of 77.8 to 53.1% were obtained.

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