Taiwan Agricultural Research Institute, Council of Agriculture, Executive Yuan--Publication-Journal of Taiwan Agricultural Research

Journal of Taiwan Agricultural Research

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Effects of Plant Growth Regulators on the Mycelia Growth and Fruit Body Formation of Pleurotus ostreatus in Liquid Medium

Ciou, S. Y., Y. Z. Zhao, Y. H. Chang, C. H. Hung, and L. R. Chen. 2017. Effects of plant growth regulators on the mycelia growth and fruit body formation of Pleurotus ostreatus in liquid medium. J. Taiwan Agric. Res. 66(3):240–247.

Effects of Plant Growth Regulators on the Mycelia Growth and Fruit Body Formation of Pleurotus ostreatus in Liquid Medium

Sian-Yi Ciou1, Yi-Zhen Zhao2, Ya-Han Chang2, Chin-Hsiung Hung3,*, and Li-Ru Chen4

Abstract

    The experiments were conducted to study the effects of plant growth regulators on the growth of mycelia and the formation of primordia and fruiting body of Pleurotus ostreatus cultured in liquid medium. The mycelia of P. ostreatus were inoculated in culture medium containing 1 mg L-1 or 6 mg L-1 α-naphthaleneacetic acid (NAA), 2,4-Dichlorophenoxyacetic acid (2,4-D), or 0.2 mg L-1 or 1.5 mg L-1 6-Benzylaminopurine (BA) at 25℃ without shaking. The results showed that the growth of mycelia in liquid medium containing 1.5 mg L-1 BA or 1, 6 mg L-1 2,4-D attained 7.5–7.8 cm after inoculation for 21 d. However, the treatment of 6 mg L-1 NAA inhibited the growth of mycelia. Twenty-one days after inoculation, treatment of 1 mg L-1 2,4-D significantly induced the formation of primordia and fruiting bodies (P < 0.05) with an avg. of 235.5 and 5.2, respectively. The application of 1.5 mg L-1 BA significantly reduced the number of primordia to the lowest (58.4 in avg.), and 6 mg L-1 2,4-D treatment inhibited the formation of fruit bodies (2.6 in avg.). Study indicated that application of 1 mg L-1 2,4-D in liquid medium can significantly enhance the growth of mycelia and the formation of primordia and fruit body of P. ostreatus. The scale-up experiments based on this study will be conducted for optimizing the commercial production of P. ostreatus in the future.

Key words: Fruit body formation, Liquid culture, Plant growth regulator, Pleurotus ostreatus.


* Corresponding author, e-mail: chhung@mail.ncyu.edu.tw
1 Graduate Student, Department of Horticultural Science, National Chiayi University, Chiayi, Taiwan, ROC.
2 Assistant, Department of Horticultural Science, National Chiayi University, Chiayi, Taiwan, ROC.
3 Professor and Head, Department of Horticultural Science, National Chiayi University, Chiayi, Taiwan, ROC.
4 Assistant Professor, Department of Horticulture and Biotechnology, Chinese Culture University, Taipei, Taiwan, ROC.

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