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Taiwan Agricultural Research Institute, Council of Agriculture, Executive Yuan--Publication-Journal of Taiwan Agricultural Research

Journal of Taiwan Agricultural Research

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The Accurate Detection Methods for the Adventitious Presence (AP) of Hybrids of Brassica napus and Brassica rapa (campestris) L. during Winter Cropping in Taiwan

Tu, Y. K., H. W. Chen, Y. C. Hung, Y. C. Lin, and B. J. Kuo. 2019. The accurate detection methods for the adventitious presence (AP) of hybrids of Brassica napus and Brassica rapa (campestris) L. during winter cropping in Taiwan. J. Taiwan Agric. Res. 68(1):69–77.

The Accurate Detection Methods for the Adventitious Presence (AP) of Hybrids of Brassica napus and Brassica rapa (campestris) L. during Winter Cropping in Taiwan

Yuan-Kai Tu1, Han-Wei Chen1, Yi-Chu Hung2, Yen-Chun Lin3, and Bo-Jein Kuo4,*

Abstract

    Brassica oilseed is a member of Brassicaceae and is one of the major oil crops along with Glycine max, Arachis hypogaea and Helianthus annuus in the world. Nowadays, many genetically modified (GM) commercialized oilseeds have been bred in order to reduce the use of pesticide and herbicide. However, GM crops may cause the gene flow problem and result in hybridization with wild species or relative species. Therefore, the GM oilseed may cause the contamination of non-GM relatives. As a result, it is necessary to establish a fast and reliable detection method to efficiently discriminate F1 hybrid derived from crossing GM with non-GM oilseed. In this study, Brassica napus from Mainland China called ‘Deza oil No.18ʼ was used as donor parent and the winter cropping Brassica rapa in Taiwan named ‘Nongxing 80 daysʼ was used as recipient parent to produce the F1 hybrids. In this study, we tested the feasibility of detection methods, including flow cytometry (FCM), lobed-leaf trait investigation and sequence-related amplified polymorphism (SRAP) marker analysis. Firstly, the aforesaind detection methods were used to examine the F1 hybrids derived from crossing by artificial pollination. Then, we verified the detection methods using F1 hybrids obtained from natural crossing in the field. The results showed that regardless of the F1 hybrid collected from artificial-crossing or natural-crossing, lobed-leaf investigation and SRAP marker analysis were able to efficiently and robustly identify the F1 hybrids. Besides, to some extent, we also found that F1 hybrids are very similar in morphology to their donor parent. Values of sensitivity, specificity, positive predictive value and negative predictive value were 100%. The lobed-leaf discrimination and SRAP marker analysis methods could be used for correctly detecting the adventitious presence (AP) of hybrids derived from crossing B. napus and B. rapa in Taiwan.

Key words: Brassica napus, Brassica rapa, Sequence-related amplified polymorphism (SRAP), Lobed-leaf trait, Interspecific hybridization.


* Corresponding author, e-mail: bjkuo@nchu.edu.tw
1 Assistant Research Fellows, Biotechnology Division, Taiwan Agricultural Research Institute, Taichung, Taiwan, ROC.
2 Research Assistant, Department of Agronomy, National Chung Hsing University, Taichung, Taiwan, ROC.
3 Research Assistant, Biotechnology Division, Taiwan Agricultural Research Institute, Taichung, Taiwan, ROC.
4 Professor, Department of Agronomy, National Chung Hsing University, Taichung, Taiwan, ROC.

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