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Taiwan Agricultural Research Institute, Council of Agriculture, Executive Yuan--Publication-Journal of Taiwan Agricultural Research

Journal of Taiwan Agricultural Research

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Factors of Colchicine Treatments on Polyploidy Induction by Using in Vitro Seedlings of Platycodon grandiflorum

Hsia, C. N., U. C. Chen, and C. Y. Tsao. 2019. Factors of colchicine treatments on polyploidy induction by using in vitro seedlings of Platycodon grandiflorum. J. Taiwan Agric. Res. 68(3):215–225.

Factors of Colchicine Treatments on Polyploidy Induction by Using in Vitro Seedlings of Platycodon grandiflorum

Chi-Ni Hsia1,*, Uei-Chern Chen2, and Chin-Yi Tsao3

Abstract

    Plants of Platycodon grandiflorum A. DC. are vastly used as traditional Chinese medicine. However, breeding of elite varieties by hybridization has been less effective because of lacking diverse genetic resources in this species. It is thought to produce polyploidy by using colchicine that could create novel genetic resources for breeding purposes. This study was conducted to investigate effects of colchicine concentration, exposure duration and colchicine used in the liquid or the solid medium on polyploidy induction by using in vitro seedlings of P. grandiflorum in different developmental stages. Ploidy of survival plantlets derived from colchicine treatments was analyzed by using a flow cytometer. Results showed that both exposure duration of colchicine and its interaction with developmental stage of seedlings had significant effects on the survival rate of explants. The best treatments with explant survival rate higher than 80% and polyploidy induction rate more than 60% were obtained either from 1.25 mM colchicine in liquid medium for 6 d exposure or from 2.50 mM colchicine in solid medium for 3 d exposure by using cotyledon stage seedlings as explants.

Key words: Jiegeng, Ploidy, Tetraploid, Colchicine, In vitro.


* Corresponding author, e-mail: hsia@tari.gov.tw
1 Research Fellow, Biotechnology Division, Taiwan Agricultural Research Institute, Taichung, Taiwan, ROC.
2 Assistant Research Fellow, Biotechnology Division, Taiwan Agricultural Research Institute, Taichung, Taiwan, ROC.
3 Contract Assistant Research Fellow, Biotechnology Division, Taiwan Agricultural Research Institute, Taichung, Taiwan, ROC.

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