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Taiwan Agricultural Research Institute, Council of Agriculture, Executive Yuan--Publication-Journal of Taiwan Agricultural Research

Journal of Taiwan Agricultural Research

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Antifungal Effect of Antagonistic Bacteria Isolated from the Froth of Spittlebug Poophilus costalis Against Phytopathogenic Fungi

Chang, S. C., H. T. Shih, J. N. Tsai, T. C. Lin, and K. H. Lu. 2019. Antifungal effect of antagonistic bacteria isolated from the froth of spittlebug Poophilus costalis against phytopathogenic fungi. J. Taiwan Agric. Res. 68(3):226–236.

Antifungal Effect of Antagonistic Bacteria Isolated from the Froth of Spittlebug Poophilus costalis Against Phytopathogenic Fungi

Shu-Chen Chang1, Hsien-Tzung Shih2, Jyh-Nong Tsai3, Tsung-Chun Lin4, and Kuang-Hui Lu5,*

Abstract

    For no fungi growing on the froth of spittlebug in field, the antifungal effect and chitinase activity of the bacteria isolated from the froth of Poophilus costalis (Walker, 1851) were analyzed in this study. Two isolates of bacteria had high antagonistic activities against Fusarium oxysporum f. sp. pisi and Colletotrichum gloeosporioides Penz were identified as Stenotrophomonas maltophilia AY and Delftia tsuruhatensis DT by 16S rDNA and chitinase gene sequences. S. maltophilia AY produced 1.1, 19.9, and 19.1 nmol p-nitrophenol min-1 mg-1 protein of exochitinase β-N-actylglucosaminidase, chitobiosidase and endochitinase, respectively. These two antagonistic bacteria may have the application potential on pathogenic control in the future.

Key words: Stenotrophomonas maltophilia, Delftia tsuruhatensis, Chitinase, Antifungal effect.


* Corresponding author, e-mail: khlu@dragon.nchu.edu.tw
1 Assistant Research Fellow, Applied Zoology Division, Taiwan Agricultural Research Institute, Taichung, Taiwan, ROC.
2 Associate Research Fellow, Applied Zoology Division, Taiwan Agricultural Research Institute, Taichung, Taiwan, ROC.
3 Associate Research Fellow, Plant Pathology Division, Taiwan Agricultural Research Institute, Taichung, Taiwan, ROC.
4 Assistant Research Fellow, Plant Pathology Division, Taiwan Agricultural Research Institute, Taichung, Taiwan, ROC.
5 Professor, Department of Entomology, National Chung Hsing University, Taichung, Taiwan, ROC.

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