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Marker-Assisted Selection of Salt Tolerance in Rice (Oryza sativa L.)
Standards used for evaluating salt-tolerance in rice seedlings. (A) When plant growth is normal, the scale of salt-tolerance is denoted 0, (B) some leaves are rolled or discolored, the scale of salt-tolerance is denoted 1, (C) some leaf tips are drying, the scale of salt-tolerance is denoted 3, (D) about 1/2 leaves are drying, the scale of salt-tolerance is denoted 5, (E) about 2/3 leaves are drying, the scale of salt-tolerance is denoted 7, (F) and whole plant nearly dead and wilted, the scale of salt-tolerance is denoted 9.
Standards used for evaluating salt-tolerance in rice seedlings. (A) When plant growth is normal, the scale of salt-tolerance is denoted 0, (B) some leaves are rolled or discolored, the scale of salt-tolerance is denoted 1, (C) some leaf tips are drying, the scale of salt-tolerance is denoted 3, (D) about 1/2 leaves are drying, the scale of salt-tolerance is denoted 5, (E) about 2/3 leaves are drying, the scale of salt-tolerance is denoted 7, (F) and whole plant nearly dead and wilted, the scale of salt-tolerance is denoted 9.

Author:Su-Chen Kuo, Chieh-Wei Kuo, Yann-Rong Lin, and Yong-Pei Wu*

Abstract:

    The salt-tolerant mutant line ‘SM61’ was crossed to IR64, an elite indica variety which is widely grown in the world, to introgress gene(s) conferring salt tolerance to ‘IR64’ as the recurrent parent by backcrossing. The progenies of each backcross generation were screened salt tolerance by the treatment of 200 mM NaCl and consequently selected by markers for background selection to recover the recurrent parent’s genome. The means of recurrent parent’s genome were 75.0, 89.0 and 96.4% in selected BC1F1, BC2F1 and BC3F1 individuals, respectively. As compared to the mean recovery genome of BC3F1 plants by conventional backcrossing was 88.4%, background selection by marker-assisted selection (MAS) was more effcient to breed salt-tolerant lines. ‘SM61’, ‘IR64’ and six BC3F4 salt-tolerant lines derived from the backcross of ‘SM61’/‘IR64’ were evaluated for salt tolerance under the treatment of 200 mM NaCl at three-leaf, fve-leaf, seven-leaf, and after maximum tillering stages. Three salt-tolerant lines, ‘ST12’, ‘ST18’ and ‘ST59’, exhibited higher tolerance to salt stress than both the donor parent and the recurrent parent did, implying that the salt-tolerant characteristic of ‘SM61’ was successfully transferred to ‘IR64’ and generated new high salt-tolerant indica lines by MAS. In addition, the grain yields of twenty salt-tolerant BC3F4 lines were produced more than 5,000 kg per hectare, indicating that these twenty salt-tolerant lines can be grown in felds for cultivation. Hopefully these salt-tolerant lines will be registered as new rice varieties and be available for rice production on saline felds locally and internationally.

Key words:Marker-assisted selection (MAS), Mutant lines, Salt-tolerant Rice

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Journal of
Taiwan Agricultural Research
Vol. 72, No.3 (2023) ISSN: 2790-086X Vol. 72, No.3 (2023)
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