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Effect of Buffer Zones on Pollen-Mediated Gene Flow of Transgenic Rice
Diagrams of the field experimental designs without (A) or with (B) buffer zones. Pollen donor ‘TNG67’ was transplanted in the central block of 5 m × 5 m, and pollen recipient ‘TNGW73’ was transplanted to the plots in 8 directions at 5 different distances in the open field of 25 m × 25 m. Beyond the plots, no plant and weed grew where is indicated by white space (A), beyond the plots, ‘TNGW73’ grew where is indicated by gray space (B).
Diagrams of the field experimental designs without (A) or with (B) buffer zones. Pollen donor ‘TNG67’ was transplanted in the central block of 5 m × 5 m, and pollen recipient ‘TNGW73’ was transplanted to the plots in 8 directions at 5 different distances in the open field of 25 m × 25 m. Beyond the plots, no plant and weed grew where is indicated by white space (A), beyond the plots, ‘TNGW73’ grew where is indicated by gray space (B).

Author:Ching-Shan Tseng, Yann-Rong Lin, Cheng-Ping Kuan, Ming-Hsing Lai, and Min-Tze Wu*

Abstract:

    In this study, the non-glutinous of phytase-transformed rice line AAN (Oryza sativa L. ssp japonica) was used as the pollen donor and the glutinous rice ‘Tainung waxy 73’ (O. sativa L. ssp. japonica cv. ‘TNGW73’) was used as the pollen recipient. The objective of this research was to study the effect of buffer zones on pollen dispersal of transgenic rice in the open fields.Results showed that the frequency of outcrossed seeds varied with the distances and directions. The frequencies of outcrossed seeds were not signifiantly different in the south and southeast directions within 7 m between buffer zones and non-buffer zones treatments. However, there were significantlydifferent frequencies of outcrossed seeds at 9-m distance between buffer zones and non-buffer zones treatments. The frequency of outcrossed seeds with buffer zone was 0.83% (south) and 0.84% (southeast) and 1.53% (south) and 1.65% (southeast) for non-buffer zones, respectively. The frequency of outcrossed seeds of plots with buffer zones was higher than those without buffer zones in the north and northeast at distance of 1, 3, and 5 m. However, the frequency of outcrossed seeds of plots with buffer zones was significantlylower than those without buffer zone beyond 7 m. This study indicated buffer zones longer than 7 m could decrease the frequency of outcrossed seeds by pollen-mediated gene flowin the directions against wind.

Key words:Transgenic rice, Pollen-mediated gen flo, Buffer zones

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Journal of
Taiwan Agricultural Research
Vol. 73, No.2 (2024) ISSN: 2790-086X Vol. 73, No.2 (2024)
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