Journal of Taiwan Agricultural Research

Author:Ting-Chin Deng*, Chin-Hui Tsai, and Fang-Yu Ning

Abstract:

    Virus-liked wasabi plant samples were collected in Alishan Township, Chiayi in April 2012. By leaf sap inoculation, local lesions were developed on Chenopodium quinoa, from which pure virus isolates were obtained through single lesion isolation. The isolates were crossly reacted with antibody to Tomato mosaic virus (ToMV) when tested by enzyme-linked antibody immunoassay (ELISA) or Western blot. Results of inoculation tests showed that the host range was mainly limited in cruciferous plants, except for Brassica napus and B. oleracea L. var. capitata cv. Chu-chiu. Total viral RNA was purified and a pair of Tobamovirus-general primers (TMVmpf and TMV3nr) was applied to conduct reverse transcription polymerase chain reaction (RT-PCR), and that resulted in producing a 688 bp DNA fragment. The nucleotide sequence of the fragment (part of the CP gene and part of MP gene) was determined. BLAST analysis of this fragment revealed the virus isolate shared the maximum nucleotide identities (99%) with Wasabi mottle virus Tochigi isolate (AB017504). Phylogenetic analysis also showed that the viral CP gene was 99.3% identical to a wasabi strain of crucifer Tobamovirus (Acc No. BAA28951), compared with 49% identities to an isolate of ToMV from Queensland, Australia (NP_078449). Therefore, the virus isolates were identified as a Wasabi mottle virus (WMoV). However, the virus was detected in 66 out of 187 wasabi plant samples collected in Alishan area and all of these positive samples were further identified as WMoV by nucleic acid analysis. This paper is the first report of WMoV occurred in Taiwan, but WMoV should have been existed in the Alishan area already.

Key words:Tobamovirus, Alishan, Tomato mosaic virus, Brassicaceae

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