Journal of Taiwan Agricultural Research

Author:Uei-Chern Chen, Jhin-Yi Tsao, and Chi-Ni Hsia*

Abstract:

    Petiole and leaf explants derived from in vitro Salvia miltiorrhiza were used for shoot regeneration in this study. The highest adventitious shoot formation rate of 100% with 3.7 shoots/explant in average was obtained from petiole segments cultured on Murashige and Skoog’s (MS) medium containing 1 mg L^-1 N⁶-benzyladenine (BA) and 0.5 mg L^-1 α-naphthaleneacetic acid (NAA) for 6 wk of culture. Callus and adventitious roots were induced from petiole and leaf segments pre-cultured on the MS medium supplemented with 1–2 mg L^-1 2,4-dichlorophenoxyacetic acid (2,4-D) for 2–3 wk followed by transferring on a hormone-free MS basal medium for a total 6 wk of culture. Calli were proliferated on the MS basal medium containing 0.25 mg L^-1 BA and 0.2 mg L^-1 2,4-D under darkness for 8 wk of culture along with few adventitious shoots were found. Proliferated calli were subcultured to the medium containing with same concentration of 2,4-D in combination with various BA concentration under light and dark condition for shoot regeneration. The highest induction number of adventitious shoots was 14.1 shoots 0.2 g^-1 callus from the medium containing 2 mg L^-1 BA under light condition. An efficient micropropagation system of Salvia miltiorrhiza by direct and indirect adventitious shoot regeneration systems were established in this study which would not only supply for plantlet production but also apply on mutation and genetic transformation studies.

Key words:Directly organogenesis, Indirectly organogenesis, Callus formation, Micropropagation

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