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Biosafety Assessment of GM Banana: The Effect on Microflora Community in Rrhizosphere Soil
Principle component analysis of EcoPlate<sup>TM</sup> carbon substrates utilized by microflora in rhizosphere and bulk soils of different banana lines. Scores of each sample for the first and second PCs were plotted. Symbol type indicates different rhizosphere soil sampling times. The soil samples were collected from the rhizosphere of each banana lines at 1 mo (triangle symbol) and 3 yr (circle symbol) after transplanting. Bulk soil samples were collected outside the rhizosphere of banana plants. The solid and dashed ellipses describe 95% confidence region of 1 mo and 3 yr samples respectively.
Principle component analysis of EcoPlateTM carbon substrates utilized by microflora in rhizosphere and bulk soils of different banana lines. Scores of each sample for the first and second PCs were plotted. Symbol type indicates different rhizosphere soil sampling times. The soil samples were collected from the rhizosphere of each banana lines at 1 mo (triangle symbol) and 3 yr (circle symbol) after transplanting. Bulk soil samples were collected outside the rhizosphere of banana plants. The solid and dashed ellipses describe 95% confidence region of 1 mo and 3 yr samples respectively.

Author:Han-Wei Chen, Jing-Wen Chen, Ching-Shan Tseng, Min-Tze Wu, and Yuan-Kai Tu*

Abstract:

    The environmental impacts on genetically modified (GM) crops are still unknown. This study aimed to explore the potential risk of pflp (plant ferredoxin-like protein) transgenic banana lines on rhizosphere microbial community and to investigate whether the gene flow occurs through horizontal gene transfer. The conventional plate counting method and the community-level physiological profiles (CLPP) method were used for evaluating rhizosphere microbial community. The results showed that most rhizosphere soil samples taken from pflp transgenic banana lines had reduced levels of Azotobacteria and nitrite oxidizing bacteria at respective 5.38–5.96 (log10 CFU g-1) one month after transplanting. However, no significant difference was found in the quantity of culturable microorganisms in rhizosphere soils 3 years after transplanting. In this study, 2,052 colonies were analyzed for horizontal gene transfer, but no nptII fragment was detected. Results of cluster analysis for CLPP showed no difference between transgenic banana lines and their non-transgenic counterparts in microflora community composition. In addition, no significant difference was observed in species diversity and evenness of rhizosphere microorganisms between GM and non-GM banana plants. We conclude that pflp transgenic banana lines may not affect the diversity of rhizosphere microflora community structure, but have slight effects on microbial community in rhizosphere during first few months after transplanting.

Key words:Genetic modified crops, Biosafety assessment, Rhizosphere, Microflora community

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Journal of
Taiwan Agricultural Research
Vol. 72, No.3 (2023) ISSN: 2790-086X Vol. 72, No.3 (2023)
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