All issues

Effect of Explant Size, Sucrose and Activated Charcoal on In Vitro Plantlet Proliferation and Growth in Amaryllis
The growth situation of <i>Hippeastrum hybridum</i> Hort. ‘Tainung No. 1’ plantlets while in vitro plantlets cultured on the medium containing various concentrations of sucrose and activated carbon for 10 wk of culture. <i>In vitro</i> plantlets with 3−5 mm bulb diameter were cultured on MS medium containing 1 mg L<sup>-1</sup> 6-benzylaminopurine (BA) and 0.1 mg L<sup>-1</sup> α-naphthalene acetic acid (NAA) with additional various concentrations of 1.5, 3.0, 6.0, 9.0 g L<sup>-1</sup> sucrose (A, B, C, D) and 0, 0.5, 1.0, 2.0 g L<sup>-1</sup> activated charcoal (marked in picture) for 10 wk of culture. Bars = 2 cm.
The growth situation of Hippeastrum hybridum Hort. ‘Tainung No. 1’ plantlets while in vitro plantlets cultured on the medium containing various concentrations of sucrose and activated carbon for 10 wk of culture. In vitro plantlets with 3−5 mm bulb diameter were cultured on MS medium containing 1 mg L-1 6-benzylaminopurine (BA) and 0.1 mg L-1 α-naphthalene acetic acid (NAA) with additional various concentrations of 1.5, 3.0, 6.0, 9.0 g L-1 sucrose (A, B, C, D) and 0, 0.5, 1.0, 2.0 g L-1 activated charcoal (marked in picture) for 10 wk of culture. Bars = 2 cm.

Author:Uei-Chern Chen, Jhin-Yi Tsao, Tzu-Ying Wu, and Chi-Ni Hsia*

Abstract:

    Factors of explant size, concentration of sucrose and activated charcoal in the culture medium were investigated on in vitro growth of Hippeastrum hybridum Hort. ‘Tainung No. 1’ plantlet in this study. One-quarter vertical cut bulb explants derived from 3, 6 and 9 mm diameters of bulb were evaluated after 8 wk and 12 wk of culture. Proliferation rate of 1.1−1.3 plantlets per one-quarter bulb explant was obtained after 8 wk of culture, and no significant difference was found among all treatments. Furthermore, plantlets with larger bulb were obtained from explants derived from explants of 9 mm treatment. However, no significant difference on bulb diameter of 6 mm and 9 mm treatments was found after extending culture time to 12 wk of culture. A factorial experiment using 1.5−9.0% sucrose in combination with 0−2.0 g L-1 activated charcoal was conducted using explants derived from plantlets with 3−5 mm diameter of bulb. The better results were obtained from 6% sucrose in combination with 1.0−2.0 g L-1 activated charcoal on increase of bulb diameter and fresh weight which were 8.0 mm bulb diameter and 1.73−1.75 g fresh weight per plantlet, respectively. It was also found that growth of plantlets was inhibited in the medium containing 9.0% sucrose. In terms of the root number of plantlet, the best results of 8.8−9.6 roots per plantlet were obtained from 6.0% sucrose in combination with 0.5−2.0 g L-1 activated charcoal, and higher than the other treatments. For the leaf number of plantlet, no significant difference was found by using 1.5−6.0% sucrose in combination with 0.5−2.0 g L-1 activated charcoal. In summary, raising sucrose concentration from 1.5% to 6.0% would increase bulb diameter, fresh weight and root number of plantlet, and adding proper concentration of activated charcoal would has a synergistic effect with sucrose.

Key words:Hippeastrum hybridum Hort., One-quarter bulb, Masspropagation, Rooting, Synergistic effect

Download:Download PDF PDF Links

  • Back
  • Top
Journal of
Taiwan Agricultural Research
Vol. 72, No.3 (2023) ISSN: 2790-086X Vol. 72, No.3 (2023)
Submit your manuscript Guide for authors
Most Viewed
  • Back
  • Top