Journal of Taiwan Agricultural Research

Author:Yi-Ting Lin, Chin-Hui Tsai, Mei-Ju Lin, and Ting-Chin Deng*

Abstract:

    A diseased sample of desert rose (Adenium obesum) cv. Dahuahua showing virus-like symptom was found in Kaohsiung in 2013. The pure virus isolate (DR-TW) was obtained by inoculation to Chenopodium quinoa through single lesion transfer. Immunoassay showed that the isolate interacted crossly with the other tobamoviruses, including Frangipani mosaic virus (FrMV), Tomato mosaic virus (ToMV), etc. After sap inoculation, local lesions were developed on leaves of Tetragonia tetragonioides, Chenopodium amaranticolor, Chenopodium quinoa, Chenopodium murale, Gomphrena globosa and Datura stramonium; and systematic mosaic was showed on plants of Nicotiana benthamiana, frangipani and desert rose. However, the pathogenicity and virulence of DR-TW was milder than ToMV to tomatoes, peppers and eggplants. A pair of tobamovirus broad-spectrum primers (TobRT up1/TobRT do2) were applied in RT-PCR to amplify a nucleic acid fragment with 568 bp. Sequence alignment revealed that the RT-PCR fragment shared more than 98% identity with Plumeria mosaic virus (PluMV) but only 73% with FrMV. Another primers were designed and used for analysis of complete genome. The full length of genomic nucleotides with 6,683 bp was determined and deposited in GenBank under accession number KX881422. A total of 188.3 kDa and 130.4 kDa of replicates, 28.7 kDa of movement protein and 19.0 kDa of coat protein, were transcribed. A pair of PluMV-specific primers (PLuMVcp-up/PLuMVcp-dw) were designed to amplify a fragment of 643 bp nucleotides by RT-PCR, by which PluMV was detectable and could be distinguished from other tobamoviruses. The FrMV specific primers (FrMVcp-up/FrMVcp-dw) were also applied to differentiate the FrMV from PluMV by RT-PCR.

Key words:Taiwan, Isolate, Tobamovirus, Frangipani mosaic virus

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