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Identification and Evaluation of Antagonistic Actinobacteria on Controlling Potato Common Scab
Effect of antagonistic actinobacteria on controlling potato common scab in a greenhouse. (A) Mock control, (B) inoculated with <i>Streptomyces europaeiscabiei</i> St1563, (C) treated with culture filtrates of 35-2 isolate and inoculated with <i>Streptomyces europaeiscabiei</i> St1563, and (D) treated with culture filtrates of 43-21 isolate and inoculated with <i>Streptomyces europaeiscabiei</i> St1563.
Effect of antagonistic actinobacteria on controlling potato common scab in a greenhouse. (A) Mock control, (B) inoculated with Streptomyces europaeiscabiei St1563, (C) treated with culture filtrates of 35-2 isolate and inoculated with Streptomyces europaeiscabiei St1563, and (D) treated with culture filtrates of 43-21 isolate and inoculated with Streptomyces europaeiscabiei St1563.

Author:Ching-Yi Lin*, Hui-Fang Ni, and Hui-Ju Lin

Abstract:

    Potato common scab is an economically important disease that is caused by several members of the genus Streptomyces in the world. As no effective pesticides and limited results of crop rotation and resistant potato cultivars for managing the disease, this study explored the possibility of using biological control. A total of 85 isolates of actinobacteria were isolated from soil from six different locations in Kaohsiung City. Cellophane paper antibiotic method and bacterial culture filtrates application were conducted to determine antagonistic activity against Streptomyces europaeiscabiei. Isolates 35-2 and 43-21 strongly inhibited S. europaeiscabiei growth in both assays. When potato plants were drenched with culture filtrates of isolates 35-2 and 43-21, respectively, significantly reduced potato common scab incidence was observed in pot assays. The disease severity was 28% after isolate 35-2 treatment and 35% after isolate 43-21 treatment. In addition, isolates 35-2 and 43-21 increased potato tubers weight in pot assays. These results suggested that isolates 35-2 and 43-21 could provide a dual benefit by decreasing potato common scab disease severity and increasing potato yield as an effective biological control agent. Based on 16S rRNA sequencing analysis, the two antagonistic isolates 35-2 and 43-21 showed a sequence identity of 100% and 99% with Streptomyces rochei and Streptomyces neopeptinius, respectively.

Key words:Potato common scab, Streptomyces spp., Biologic control

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