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Biological Control of Potato Bacterial Wilt Caused by Ralstonia solanacearum with Bacillus amyloliquefaciens Ba01
Identification of <i>Ralstonia solanacearum</i> isolates. (A) The DNA fragments 282 and 1,070 bp of <i>R. solanacearum</i> were amplified by <i>R. solanacearum</i> specific primer pair AU759/760 and its race 1 specific primer pair PSIS-F/R, respectively, and (B) the DNA fragment 306 bp was amplified by <i>R. solanacearum</i> race 3 specific primer pair 630/631. (1 = RSN245, 2 = RSN371, 3 = RSN373, 4 = RSN439)
Identification of Ralstonia solanacearum isolates. (A) The DNA fragments 282 and 1,070 bp of R. solanacearum were amplified by R. solanacearum specific primer pair AU759/760 and its race 1 specific primer pair PSIS-F/R, respectively, and (B) the DNA fragment 306 bp was amplified by R. solanacearum race 3 specific primer pair 630/631. (1 = RSN245, 2 = RSN371, 3 = RSN373, 4 = RSN439)

Author:Zen-Chi Huang, Ya-Fang Wu, Chia-Hsin Tsai, Hau-Ping Chou, Yi-Hsien Lin, and Ying-Lien Chen*

Abstract:

    Solanaceae bacterial wilt caused by Ralstonia solanacearum has been an important disease of potato and tomato. Though there were no recommended bactericides, current ways to control the bacterial wilt included using bacteria-free tubers, rotation with cereals or rice, and applying Bacillus amyloliquefaciens PMB01 as a biocontrol agent. B. amyloliquefaciens Ba01 was isolated from healthy potato tuber, and demonstrated the antibacterial effect against potato common scab. In this study, we found that B. amyloliquefaciens Ba01 effectively inhibited the growth of potato bacterial wilt pathogen R. solanacearum, and it can enhance phosphate solubilization and secrete multiple extracellular enzymes, such as protease, amylase, cellulase and lipase, which could facilitate the growth of plants. Meanwhile, Ba01 exerted better swimming/swarming ability compared to PMB01, indicating it might colonize well in potato plants. We also found that Ba01 had better ability in biofilm formation than PMB01. In terms of antibacterial activity, both Ba01 and PMB01 can inhibit R. solanacearum race 1 strain RSN371, and race 3 strains RSN245, RSN373 and RSN439, but there were no synergistic effect between these two biocontrol agents. Furthermore, RSN439 has stronger virulence to potato among four R. solanacearum strains. Hence, we determined the disease severity of potato inoculated with R. solanacearum RSN439, and found that the disease severity significantly reduced to 20% (P = 0.0009) or 40% (P = 0.0135) after treatment with Ba01 or PMB01, respectively. As for plant defensive responses, leaves obtained from potato plants inoculated with R. solanacearum RSN439 and Ba01 or PMB01 simultaneously increased the accumulation of reactive oxygen species (ROS). In conclusion, potato bacterial wilt can be controlled by B. amyloliquefaciens Ba01 effectively due to the inhibition of R. solanacearum, the secreting of extracellular enzymes, phosphate solubilization and ROS accumulation in potato plants.

Key words:Potato bacterial wilt, Ralstonia solanacearum, Bacillus amyloliquefaciens Ba01, Biological control

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Journal of
Taiwan Agricultural Research
Vol. 73, No.1 (2024) ISSN: 2790-086X Vol. 73, No.1 (2024)
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