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Effects of Rhizome Diameter and Two-Stage Continuous Culture on Proliferation Rate and Growth of In Vitro Zingiber officinale Plantlets
Shoot-bud clusters and plantlets of <i>in vitro Zingiber officinale</i> ‘Chu’ derived from media with various benzylamino-purine (BA) concentrations and culture durations. <i>In vitro</i> rhizomes (about 5 mm in diameter) were cultured in a MS liquid medium supplemented with 0.1 mg L<sup>-1</sup> NAA and in combined with (A) 0, (B) 1.0 mg L<sup>-1</sup> BA for 2 wk, (C) 0.5, (D) 2.0 mg L<sup>-1</sup> BA for 4 wk and (E) 0.5, (F) 1.0 mg L<sup>-1</sup> BA for 6 wk of culture. YS: yellow shoot-bud, GS: green shoot-bud, and PL: plantlet with unfolded leave. Bars = 1 cm.
Shoot-bud clusters and plantlets of in vitro Zingiber officinale ‘Chu’ derived from media with various benzylamino-purine (BA) concentrations and culture durations. In vitro rhizomes (about 5 mm in diameter) were cultured in a MS liquid medium supplemented with 0.1 mg L-1 NAA and in combined with (A) 0, (B) 1.0 mg L-1 BA for 2 wk, (C) 0.5, (D) 2.0 mg L-1 BA for 4 wk and (E) 0.5, (F) 1.0 mg L-1 BA for 6 wk of culture. YS: yellow shoot-bud, GS: green shoot-bud, and PL: plantlet with unfolded leave. Bars = 1 cm.

Author:Uei-Chern Chen, Chin-Yi Tsao, Tzu-Ying Wu, and Chi-Ni Hsia*

Abstract:

    The influence of rhizome diameter and liquid-solid two-stage continuous culture on proliferation rate and growth of in vitro Zingiber officinale ‘Guang Dong’ and ‘Chu’ plantlets were investigated in this study. The rhizomes of in vitro plantlets with about 2, 4 and 6 mm rhizome diameter were cultured on a Murashige & Skoog (MS) solid medium supplemented with 1 mg L-1 benzylaminopurine (BA) and 0.1 mg L-1 α-naphthalene acetic acid (NAA) for 8 wk of culture. There were 6.2–6.7 plantlets were induced from per explant of ‘Guang Dong’ rhizome, and no significant difference among three size of rhizomes. There were 6.8–6.9 plantlets were obtained from per 4 mm and 6 mm explants which were significantly higher than that of from 2 mm rhizome of ‘Chu’. Moreover, the rhizome diameter larger than 4 mm was considered better explants for growth of the plant height and fresh weight. In the two-stage continuous culture, in vitro rhizomes with 5 mm diameter were first culture in a liquid medium containing 0.5 mg L-1 BA and 0.1 mg L-1 NAA for 2, 4 or 6 wk of culture before subculturing on a solid medium containing 0.5 mg L-1 BA and 0.1 mg L-1 NAA for 8 wk of culture. The highest number of proliferated plantlets were obtained from the liquid medium containing 2.0 mg L-1 BA or 0.5–2.0 mg L-1 BA for 6-wk treatment in ‘Guang Dong’ and ‘Chu’, respectively. In short conclusion, the diameter of rhizome larger than 4 mm as explants would be beneficial on plantlet proliferation and growth. In according to proliferation efficiency, explants first cultured in a liquid medium containing with 2.0 mg L-1 BA in ‘Guang Dong’ and with 1.0–2.0 mg L-1 BA in ‘Chu’ for 4 wk of culture before subculturing on the solid medium for 8 wk of culture had the highest plantlet yield on a weekly basis of production.

Key words:Zingiber officinale, Micropropagation, In vitro rhizome, Liquid culture, Two-stage continuous culture

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