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Development of Real-Time Reverse TranscriptionPolymerase Chain Reaction to Identify and Detect Potato Virus Y and Pepper Mottle Virus in Sweet Pepper
Fig. 2.　Sensitivity detection of reverse transcription-polymerase chain reaction (RT-PCR) for both potatovirus Y (PVY) and pepper mottle virus (PepMoV). Mixed with PVY and PepMoV, 10× serial dilutions of total RNA as templates (1–10-7 ng). Lane M: 100 bp marker
Fig. 2.　Sensitivity detection of reverse transcription-polymerase chain reaction (RT-PCR) for both potatovirus Y (PVY) and pepper mottle virus (PepMoV). Mixed with PVY and PepMoV, 10× serial dilutions of total RNA as templates (1–10-7 ng). Lane M: 100 bp marker

Author:Cheng-Ping Kuan*, Ying-Huey Cheng, Chia-Hsin Tsai, Ching-Shan Tseng, and Tso-Chi Yang

Abstract:

The real-time reverse transcription-polymerase chain reaction (RT-PCR) method was, for the first, developed to identify and differentiate potato virus Y and pepper mottle virus in sweet pepper. The results showed that this nucleic acid amplification technology can simultaneously detect both potato virus Y and pepper mottle virus. No amplification was obtained from other Solanaceae viruses and healthy plants. The method can be used to identify single or multiple infections. The results indicated that this quantitative RT-PCR has the advantages of good specificity and high sensitivity. The technology will be applied to assess field surveillance for sweet pepper. 

Key words: Potato virus Y, Pepper mottle virus, Detection, Real-time RT-PCR.

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Journal of
Taiwan Agricultural Research
Vol. 72, No.3 (2023) ISSN: 2790-086X Vol. 72, No.3 (2023)
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