Journal of Taiwan Agricultural Research

Author:Meei-Hsing Chen, Jin-Torng Peng and Jene-Tung Chen*

Abstract:

    Four strains of Lepista nuda from France (INRA) were cultivated on a mushroom compost for selection of suitable parental strains for culture improvement. The strain LNE2 was found to be the most productive among them. There were no significant difference in productivity between the strains LNG and LNP. However, the fruiting bodies of the former strain were larger than those of the latter one. Therefore the strains LNE2 and LNG were selected for collection of basidiospores and single spore isolation. Five and thirty monosporous isolates were thus obtained from the strains LNE2 and LNG respectively. Inter-strain mating of the monosporous isolates of the latter two strains resulted in the formation of 150 hybrid dikaryons which were subjected to a series of fruiting tests. Two hybrid dikaryons, LA82 and LA84, were found to be the most productive strains, producing 2308g and 2365g/l5kg mushroom compost with a biological efficiency of 40.71% and 47.26% respectively. It took 39.8 and 43.0 days from casing soil to the first harvest for the strain LA82 and LA84 respectively.

Key words:Culture improvement, Lepista nuda, Compost

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