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Effect of Glutathione on In Vitro Proliferation and Ex Vitro Growth of Hippeastrum hybridum Plantlets
Proliferation and growth of <i>in vitro Hippeastrum hybridum</i> 'Red Lion' plantlets. One-quarter bulb was immersed in 32.50 μM and 65.00 μM reduced glutathione (GSH) (A1, A2), 16.25 μM and 32.50 μM oxidized glutathione (GSSG) (B1, B2) or autoclaved water (C) for 1 h (a) or 2 h (b), before inoculated on a MS (Murashige & Skoog 1962) medium containing 3% sucrose, 1.0 mg L<sup>-1</sup> 6-benzylaminopurine (BA) and 0.1 mg L<sup>-1</sup> 1-naphthylacetic acid (NAA) under light (38 μmol m<sup>-2</sup> s<sup>-1</sup>) (L) or dark (D) condition for 8 wk of culture. Bar = 1 cm.
Proliferation and growth of in vitro Hippeastrum hybridum 'Red Lion' plantlets. One-quarter bulb was immersed in 32.50 μM and 65.00 μM reduced glutathione (GSH) (A1, A2), 16.25 μM and 32.50 μM oxidized glutathione (GSSG) (B1, B2) or autoclaved water (C) for 1 h (a) or 2 h (b), before inoculated on a MS (Murashige & Skoog 1962) medium containing 3% sucrose, 1.0 mg L-1 6-benzylaminopurine (BA) and 0.1 mg L-1 1-naphthylacetic acid (NAA) under light (38 μmol m-2 s-1) (L) or dark (D) condition for 8 wk of culture. Bar = 1 cm.

Author:Uei-Chern Chen, Jhin-Yi Tsao, Tzu-Ying Wu, and Chi-Ni Hsia*

Abstract:

    Effects of glutathione on in vitro proliferation and ex vitro growth of plantlets of Hippeastrum hybridum Hort. ‘Red Lion’ or ‘Blossom Peacock’ were investigated in this study. One-quarter in vitro bulb of ‘Red Lion’ was taken as an explant treating with various concentrations of reduced (GSH) or oxidized (GSSG) glutathione for 1 h or 2 h before culturing on a MS solid medium supplemented with 3% sucrose, 1.0 mg L-1 benzylaminopurine (BA) and 0.1 mg L-1 α-naphthalene acetic acid (NAA) for 12 wk under 38 μmol m-2 s-1 light condition. The best proliferation rate of 14.7 plantlets per bulb was obtained by using 65.00 μM GSH solution for 2 h shaking. In vitro one-quarter bulb of ‘Blossom Peacock’ used as explant was cultured in a liquid medium containing various concentration of GSH or GSSG under 10 μmol m-2 s-1 light condition for various duration. Result showed that the highest proliferation rates were from 65.00 μM GSH treatment in a 4-wk-span culture and no significant difference was found among all glutathione treatments with the control after 8-wk culturing. However, prolonging culture time to 12 wk, the highest proliferation rate of 8.7 plantlets per bulb was obtained from 16.25 μM GSSG treatment. The effect of glutathione on growth was conducted using the 9-wkold de-flask plantlets of ‘Red Lion’ in either 12 mm or 8 mm bulb diameter by spraying with various concentrations of GSH and GSSG solution every 4 wk for consecutive five times in greenhouse. Growth data of plants was collected at 7 wk after the last glutathione application. Results showed that larger plantlets (with 12 mm bulb diameter) sprayed with 162.80 μM GSSG having higher fresh weight on leaves and whole plant than that of the control. In respect of the smaller plantlets (with 8 mm bulb diameter), higher whole plant weights were found from treatments of 162.80 μM and 325.70 μM GSSG than that of the control. In conclusion, in vitro explants of ‘Red Lion’ treated with 65.00 μM GSH solution for 2 h were found to have the highest proliferation rate. In regard to ex vitro plantlet growth, the 9-wk-old de-flask plantlets of ‘Red Lion’ spraying with 162.80 μM GSSG solution at 4 wk interval for 5 times in total had the largest bulb diameter and fresh weight.

Key words:Amaryllis, Reduced glutathione, Oxidized glutathione, Illumination, Acclimation

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Journal of
Taiwan Agricultural Research
Vol. 73, No.2 (2024) ISSN: 2790-086X Vol. 73, No.2 (2024)
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