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![In vitro rooting and acclimatization of F. hupehensis bulblets. (a) Rooting of bulblets in 1/2 MS basal medium supplemented with 0.5 mg/l BA+4 mg/l NAA, under light intensity in 2, 000 lux for 2 months. Bar, 5 mm. (b) In vitro propagated plantlets were transplanted to soil, peat moss, and sand (v/v/v=2/1/1), and cultured in growth chamber for 3 months. Bar, 5 mm.](../../df_ufiles/g/4-1(19).jpg)
Author:Uei-Chin Chen, Chung-Der Tai, Chung-Chuan Chen, and Hsin-Sheng Tsay
Abstract:
Bei-Mu (Fritillaria hupehensis Hsiao et K. C. Hsia) has been used as antitussive and expectorant in traditional Chinese medicine. As the amount of bulb collected from naturally-grown and traditionally-cultured plants is far from enough for medicinal demand, therefore the mass propagation method using bulblets of F. hupehensis has been established. α-Naphthaleneacetic acid (NAA) in concentration range of 2-4 mg/l could promote the root initiation of the bulblets. The bulblets cultured on medium containing 1/2 Murashige and Skoog (MS) medium, 3% sucrose, 0.5 mg/l 6-benzylaminopurine (BA), and 4 mg/l NAA for 60 days, consequently, 65% bulblets could develop good root system. The in vitro propagated bulblets with well-developed roots could be transplanted to autoclaved soil, peat moss, and sand (v/v/v=2/l/1), and grown well in a growth chamber. The survival percentage of bulblets was 90% by one month of culture.
Key words:Fritillaria hupehensis, Medicinal plant, Tissue culture, In vitro rooting, Acclimatization
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