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Studies on the Tissue Culture of Fritillaria hupehensis Hsiao et K. C. Hsia
Ⅲ. Influence of Medium Component and Light Treatment on in vitro Rooting, and Acclimatization of Bulblet
In vitro rooting and acclimatization of F. hupehensis bulblets. (a) Rooting of bulblets in 1/2 MS basal medium supplemented with 0.5 mg/l BA+4 mg/l NAA, under light intensity in 2, 000 lux for 2 months. Bar, 5 mm. (b) In vitro propagated plantlets were transplanted to soil, peat moss, and sand (v/v/v=2/1/1), and cultured in growth chamber for 3 months. Bar, 5 mm.
In vitro rooting and acclimatization of F. hupehensis bulblets. (a) Rooting of bulblets in 1/2 MS basal medium supplemented with 0.5 mg/l BA+4 mg/l NAA, under light intensity in 2, 000 lux for 2 months. Bar, 5 mm. (b) In vitro propagated plantlets were transplanted to soil, peat moss, and sand (v/v/v=2/1/1), and cultured in growth chamber for 3 months. Bar, 5 mm.

Author:Uei-Chin Chen, Chung-Der Tai, Chung-Chuan Chen, and Hsin-Sheng Tsay

Abstract:

Bei-Mu (Fritillaria hupehensis Hsiao et K. C. Hsia) has been used as antitussive and expectorant in traditional Chinese medicine. As the amount of bulb collected from naturally-grown and traditionally-cultured plants is far from enough for medicinal demand, therefore the mass propagation method using bulblets of F. hupehensis has been established. α-Naphthaleneacetic acid (NAA) in concentration range of 2-4 mg/l could promote the root initiation of the bulblets. The bulblets cultured on medium containing 1/2 Murashige and Skoog (MS) medium, 3% sucrose, 0.5 mg/l 6-benzylaminopurine (BA), and 4 mg/l NAA for 60 days, consequently, 65% bulblets could develop good root system. The in vitro propagated bulblets with well-developed roots could be transplanted to autoclaved soil, peat moss, and sand (v/v/v=2/l/1), and grown well in a growth chamber. The survival percentage of bulblets was 90% by one month of culture.

Key words:Fritillaria hupehensis, Medicinal plant, Tissue culture, In vitro rooting, Acclimatization

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Journal of
Taiwan Agricultural Research
Vol. 73, No.1 (2024) ISSN: 2790-086X Vol. 73, No.1 (2024)
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