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Identification of Interspecific Hybrids from Crosses between Brassica oleracea and B. campestris
An interspecific hybrid plant (Brassica campestris ssp. chinensis × Brassica oleracea var. aboglabra) (B). Note leaf morphology is different among the hybid plant (A, center) and its female parent, B. campestris (mock pak-choi) (A, left) and male parent, B. oleracea (Chinese kale) (A, right).
An interspecific hybrid plant (Brassica campestris ssp. chinensis × Brassica oleracea var. aboglabra) (B). Note leaf morphology is different among the hybid plant (A, center) and its female parent, B. campestris (mock pak-choi) (A, left) and male parent, B. oleracea (Chinese kale) (A, right).

Author:Chin-Chun Chiou, Tzu-Kai Lin*, Chen-Yu Lin, Chi-Ni Hsia, and San-Tai Wang

Abstract:

    In some crops, inter-specific hybridization is more useful than intra-specific hybridization for improvement of crop yield and quality.  The objective of this study was to develop interspecifc hy-brids using different lines of Brassica oleracea var. aboglabra and B. campestris ssp. chinensis.  After reciprocal bud pollination, imature hybid embryos were collected and cultured on the modifed Mu-rashige and Skoog’s medium.  Results showed that the effciency of producing interspecifc hybrids was affected by embryo stage, genotype and medium composition.  The effciency of embryo rescue was affected by genotype and more interspecifc hybrids were produced when B. campestris was used as female.  The phenotype and relative amount of DNA in hybrids were between their parents but these characteristics were affected more by female parent than male parent.

Key words:Brassica oleracea var. alboglabra, Brassica campestris ssp. chinensis, Interspecifc hy-bridization, Embryo rescue

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Journal of
Taiwan Agricultural Research
Vol. 72, No.3 (2023) ISSN: 2790-086X Vol. 72, No.3 (2023)
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