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Studies on Fruits Internal Browning of Pineapple (Ananas comosus (L.) Merr.) by Harvest Method
Effects of harvesting methods on polyphenol oxidase (PPO) activities of pineapple fruits (cultivar TN17) in postharvest storage.
Effects of harvesting methods on polyphenol oxidase (PPO) activities of pineapple fruits (cultivar TN17) in postharvest storage.

Author:Pai-Chi Syue, Chia-Hui Tang, and Tan-Cha Lee*

Abstract:

    Although both hand-harvest and sickle-harvest methods are used for harvesting pineapple fruits in Taiwan, the hand-harvest method is more popular than the sickle-harvest method. However, unlike the smooth cutting surface of the fruit stem by sickle-harvesting, pineapple fruits harvested by hands often resulted in uneven breaks of the stem, creating large wounds with rough surface and, thereby, reduced storage or shelf-life of the harvested produce. Results of this study showed that all the hand-harvested pineapple fruits stored at cold room (12.5℃) for 2 weeks and then moved them onto shelf (25℃) for 4 days, developed severe internal browning of fruit tissues (browning intensity index of 5.8 or >51% of browning tissues) and of no market values; whereas the internal browning of the sickle-harvested fruits was mild (browning intensity index of 2.0 or 3-5% of browning tissues) for the same storage period and conditions. The phenylalanine ammonia lyase (PAL) in harvested pineapple fruits increased with time of storage but the level of PAL in hand-harvested fruits was significantly higher than sickle-harvested ones. The polyphenol oxidase (PPO) and peroxidase (POD) in pineapple fruits decreased with time of storage but there was no significant difference between hand-harvest and sickle-harvest treatments.

Key words:Hand-harvesting, Sickle-harvesting, Phenylalanine ammonia lyase, Polyphenol oxidase, Peroxidase, Internal browning

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