Journal of Taiwan Agricultural Research

Author:Ien-Chie Wen*, Ching-Yi Chang, and Chien-Yuan Kao

Abstract:

    Alishan flowering cherry (Prunus transarisanensis Hayata) is an endemic flowering cherry species of Taiwan.  In Flora of Taiwan, Alishan flowering cherry only the whitish or light pink flowers had been recorded.  In this study, we collected Alishan flowering cherry with white, whitish pink, and pink flowers.  The color of new leaf and leaf shape also showed difference.  It demonstrated that Alishan flowering cherry had broadly genetic diversity.  The genetic relationships of 15 flowering cherry cultivars were analyzed by using RAPD markers.  A total of 300 primers were screened for their ability to produce strongly amplified products.  Out of 13 primers amplified 121 DNA bands, 89 of which were polymorphic (73.6%).  A dendrogram based on the UPGMA cluster analysis was constructed.  All tested flowering cherry cultivars were divided into seven groups, including 3 groups of Alishan flowering cherry cultivars and 4 individual cultivars.  Wu-ling No.2 and Wu-ling No.3 had 88% similarity indexes, indicating the closest genetic relationship.  Nevertheless, Wu-sheh Ying (Prunus taiwaniana Hayata) having 39% similarity indexes showed the farest genetic relationship with other tested Alishan flowering cherry cultivars.

Key words:Prunus transarisanensis, Genetic diversity, RAPD

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