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Characterization of Novel Strains of Citrus Canker Bacterium from Citrus in Taiwan
Symptoms in duced on leaves of Mexican lime (A, B, C) and garapefruit (D, E, F) by strains of X. axonopodis pv. citri 24 days after inoculation. (A and D): Typical erumpent canker lesions with watersoaked margin and light chlorotic halo induced by type Xac-A strain XW47, (E): Flat necrosis lesions with watersoaked margin and light chlorotic halo induced by type Xac-A strain XW47, (C and F): Flat necrosis lesions with watersoaked margin and light chlorotic halo induced by type Xac-A strain XL38. (Bars = 1 mm).
Symptoms in duced on leaves of Mexican lime (A, B, C) and garapefruit (D, E, F) by strains of X. axonopodis pv. citri 24 days after inoculation. (A and D): Typical erumpent canker lesions with watersoaked margin and light chlorotic halo induced by type Xac-A strain XW47, (E): Flat necrosis lesions with watersoaked margin and light chlorotic halo induced by type Xac-A strain XW47, (C and F): Flat necrosis lesions with watersoaked margin and light chlorotic halo induced by type Xac-A strain XL38. (Bars = 1 mm).

Author:Hsin-Cheng Lin*, Hsiang Chang, and Kuo-Ching Tzeng

Abstract:

    Two strains of Xanthomonas axonopodis pv. citri isolated from citrus plants in Taiwan were examined for pathogenicity on the leaves of Mexican lime (Citrus aurantifolia), grapefruit (C. paradisi), Liucheng (C. sinensis), and lemon (C. limon).  The results showed that two strains induced flated necrotic lesions with watersoaked margin on the leaves of four Citrus species.  Based on physiological, biochemical, genetic and proteinic characterizations including NaCl tolerance, hydrolysis of gelatin, oxidation of carbon sources, polymerase chain reactions with primers specific to X. axonopodis pv. citri (Xac), rep-PCR, and SDS-PAGE analysis, these two strains were identified as Xac and grouped into Xac-Ap type.  Both strains could be differentiated from X. axonopodis pv. citrumelo (pathotype E) by oxidation of carbon sources, pectolytic activity, and amplified DNA profiles of PCR, while they could be only differentiated from type Xac-Af strain by a distinct pathogenicity on the leaves of Mexican lime.  Our data indicated they appeared to be novel strains of Xac in Taiwan.  We designated these two atypical symptoms-inducing strains as Xac-Ap type which are different from strains of types Xac-A , Xac-Af, Xac-Ar, Xac-A* and Xac-Aw described previously.

Key words:Citrus canker, pthA gene, Rep-PCR, SDS-PAGE, Xanthomonas axonopodis pv. Citri

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Journal of
Taiwan Agricultural Research
Vol. 73, No.1 (2024) ISSN: 2790-086X Vol. 73, No.1 (2024)
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