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In Vitro Seed Germination and Mass Propagation of Dendrobium moniliforme, a Native Species in Taiwan
Native dendrobium moniliforme (A) plants collected from Chi-Lan mountain, I-Lan, Taiwan, (B) a cylindrical stem with long, narrow leaves, (C) a flower showing white petals and a lip petal with a brown colored band. (bar = 1.0 cm)
Native dendrobium moniliforme (A) plants collected from Chi-Lan mountain, I-Lan, Taiwan, (B) a cylindrical stem with long, narrow leaves, (C) a flower showing white petals and a lip petal with a brown colored band. (bar = 1.0 cm)

Author:Shu-Fang Lo, Chao-Lin Kuo, Chung-Li Chen, and Hsin-Sheng Tsay*

Abstract:

    An in vitro study was conducted to examine seed germination Dendrobium moniliforme harvested at different maturity stages on three different media.  Results showed that the maximum number of germination rates and fresh weight of plantlets were obtained when results showed that the treatment of 12-week-old seeds on the 1/2 MS  medium supplemented with 3% sucrose and 0.9% Difco agar was the best for seed germination and fresh weight of plantlets.  Seeds on the other two media, KC and VW, produced only albino plantlets.  The maximum biomass was obtained when the young seedlings were subcultured on the MS medium supplemented with 3–4.5% sucrose, but these seedlings were unsuitable for transplanting due to turn upward roots.  However, the medium containing MS basic salt, 1.5% sucrose, 8% each of banana homogenate, potato juice and coconut milk and 0.9% Difco agar was suitable for maximum production of biomass.

Key words:Dendrobium moniliforme, In vitro seed germination, Mass propagation

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Journal of
Taiwan Agricultural Research
Vol. 73, No.2 (2024) ISSN: 2790-086X Vol. 73, No.2 (2024)
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