Journal of Taiwan Agricultural Research

Author:Chin-Chih Chen*, Ting-Chin Deng, Chun-Huei Huang, Bi-Yun Lin, and Jye-Yann Liao

Abstract:

    A virus isolate-WF was isolated from lettuce leaves that exhibit yellowing mosaic.  WF isolate was positively reacted with potyvirus-specific monoclonal antibody.  A 1.3-kb DNA fragment was amplifed from RNA of WF isolate with the potyvirus-degenerate primer pair (HRP-5/Oligo-dT₍₁₄₎) by reverse transcription- polymerase chain reaction (RT-PCR).  The identity of deduced amino acid sequence of coat proteins of WF isolate to that of Bidens mottle virus sunfower isolate SF-1 (GenBank Acc. NC_014325) was 99.3%.  When the antiserum against purifed virions of WF isolate was used in SDS-immunodiffusion, identical immunoprecipitation reaction was generated between lettuce isolates WF and the typical isolate of BiMoV (ATCC PV165).  WF isolate could be recognized as a strain of BiMoV based on the results of the molecular and serological characterizations. Another lettuce isolate (HL) from Hualien and three isolates from Bidens pilosa (B3, B4, B12) were also serologically identical to isolate of BiMoV-PV165 and the identity in amino acid sequences of viral coat proteins were 97%.  Full-length of genomic RNAs of BiMoV-WF, HL, B3, B4, and B12 were sequenced depossited into GenBank (accession numbers EU250213, EU250214, EU250212, EU250211, and EU25021, respectively).  The phylogenetic analyses based on the deduced amino acid sequences of the full polyproteins with those of different BiMoV isolates revealed that isolates from the same host was classifed into the same group.  This is the frst report of the full-length genomic sequence and molecular characterization of BiMoV infecting lettuce and bidens.

Key words:Bidens mottle virus, Lettuce, Full-length genomic sequences

Download: PDF Links