Journal of Taiwan Agricultural Research

Author:Hui-Ling Lo, Shough-Peng Lee, Xiu-Ping Chen, De-Lin Cai, Jaw-Neng Lin, San-Tai Wang, Kan-Shu Chen*, and Yau-Wen Yang*

Abstract:

      This study was to investigate genetic variation of caulifower (Brassica oleracea var. botrytis), using 16 commercial hybrid cultivars developed in Taiwan and their parental lines and two open pollinated lines; and to fnd molecular markers which can be used for testing F₁ seed parity.  Seven oligomers from initial screening were selected and used to generate 92 polymorphic molecular markers for studying phylogenetic relationships among these 34 caulifower Cultivars/lines from three seed companies.  The phylogenetic tree of the 34 caulifower Cultivars/lines showed that seed source was an important factory affecting genetic relationships of caulifower, Cultivars/lines from same company were more closely related then Cultivars/lines from different companies.  Moreover, more than 30 molecular markers were present in F₁ hybrids and male parent but were absent in female parent.  These molecular markers can be used to test the purity of F₁ seeds of caulifower and, are of potential for commercial production of hybrid cultivars.

Key words:Caulifower, Variety, Genetic diversity, Molecular marker

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