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Evaluation of Reproduction of Pratylenchus coffeae on Yam (Dioscorea spp.) in vitro Culture
In vitro cultures of yam cv. Tainung No.5 on B5 medium for 2 months (A) and Pratylenchus coffeae on calli of carrot (B).
In vitro cultures of yam cv. Tainung No.5 on B5 medium for 2 months (A) and Pratylenchus coffeae on calli of carrot (B).

Author:Hui-Fang Ni, Su-Yu Lai, Shu-Li Hsu, Shu-Fen Chang, and Hong-Ren Yang*

Abstract:

    Pratylenchus coffeae is a major pathogenic nematode causing severe yield losses of yam (Dioscorea spp.) in Taiwan.  The objective of this study was to evaluate reproduction of P. coffeae on yam in vitro culture.  Female P. coffeae cultured on carrot callus tissues were inoculated on yam roots, cv. Tainong No.5, Chungpu 018, and Shanlin 067 in test tubes.  After incubation at 25℃ under a 12 h light-12 h dark regime for 15 weeks, number of nematodes in each tube was determined.  Results showed that population of P. coffeae differed significantly (P < 0.05) among the yam cultivars, high (8163 nematodes) in cv. Tainong No.5 and low (1551 nematodes) in cv. Shanlin 067.  The difference in population of P. coffeae between cv. Tainong No.5 and cv. Shanlin 067 was further confirmed by the inoculation of plants grown in pots under greenhouse conditions.  This study suggests that the in vitro culture method is useful for testing yam cultivars or lines for population dynamics of P. coffeae.

Key words:Yam, Dioscorea spp., Nematode, Pratylenchus coffeae, Reproduction, In vitro culture

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Journal of
Taiwan Agricultural Research
Vol. 73, No.1 (2024) ISSN: 2790-086X Vol. 73, No.1 (2024)
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