Journal of Taiwan Agricultural Research

Author:Uei-Chern Chen, Choi-Yi Lee, Hsiao-Sung Chan, Chin-Yi Tsao, and Chi-Ni Hsia*

Abstract:

    The objective of this study was to examine the effect of metal ions and elicitors on root growth and tanshinones production of Salvia miltiorrhiza hairy root culture.  Metal ions of Cu²⁺, Fe²⁺, Mn²⁺, and Co²⁺ with various concentrations were tested.  Results showed except Co²⁺ treatment that Fe²⁺, Mn²⁺, and Cu²⁺ were all able to increase the dry weight of hairy roots, significantly comparing the control treatment.  Moreover, applications of suitable concentration of Fe²⁺, Mn²⁺ and Co²⁺ into the medium could promote tanshinones accumulation.  Among them, the 100 μM Fe2+ treatment the highest yield of tanshinones had a 1.8-fold increase than that of the control.  Elicitors of yeast elicitor, jasmonic acid, abscisic acid and salicylic acid were found no effect on root growth however only the first three could enhance tanshinones production.  Among elicitor treatments, best result with a 3.4-fold increase on tanshinones production was found by using 2 mg/L ABA than the control.  Consecutive experiment using 1, 2, and 4 mg/L ABA in combination with expose duration was conducted.  All ABA treatments resulted in higher tanshinones production than that of the control.  The highest tanshinones production 22.9 mg/L was found using 1 mg/L ABA for 3-weeks exposure duration and cryptotanshinone and tanshinone IIA were counted for the major increasing part of the yield.

Key words:Danshen, Salvia miltiorrhiza, Metal ion, Elicitator, Abscisic acid (ABA), Yeast elicitor (YE), Secondary metabolites, Tanshinones, Hairy root culture

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