Journal of Taiwan Agricultural Research

Author:Shu-Chin Lo and Hsin-Sheng Tsay*

Abstract:

    This study was conducted to determine effects of auxins [NAA (α-naphthaleneacetic acid), IAA (indole-3-acetic acid) and IBA (indole-3-butyric acid)] and ventilation on in vitro micropropagation of Mesona procumbens Hemsl.  Shoots of M. procumbens were grown in dark on 1/2 MS media supplemented with 0.5 mg/L of NAA for 4 weeks for formation of granule-like calluses and then placed under light for another 4 weeks for the development of plantlets.  The plantlets were cut into segments, transferred on 1/2 MS media supplemented with 0.5 mg/L of NAA, IAA or IBA, and incubated at 25℃under light 30–35 mol m^-2 s^-1 for 6 weeks to compare effects of auxins on plantlet growth and development of plantlets.  In another experiment, plantlets were transferred on 1/2 MS media supplemented with 0.5 mg/L of IBA to study effect of different container sealing materials and ventilation closures on hyperhydricity of plantlets.  Results showed that among the three auxins tested, the medium containing 0.5 mg/L of IBA was most suitable for growth of plantlets of M. procumbens with a root weight of 0.031g/plant, compared to 0.011g/plant for the treatment of IAA.  Ventilation affected growth of plantlets.  When the tissue culture containers were sealed with dispense paper and Parafilm for 5 weeks and then removed the Parafilm for ventilation, the survival rate of ex vitro acclimation of plantlets increased to 98.7%, compared to 92.1% in the treatment of plantlets in aluminum foil.  Thus, it is possible to produce healthy plantlets of M. procumbent in tissue culture with proper amount of IBA in the growth medium and proper control of ventilation.  The protocol established in this study would be useful for in vitro propagation of M. procumbent and it could be a potential alternative to the conventional propagation of this crop in the field.

Key words:Mesona procumbens, Tissue culture, Auxins, Ventilation

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