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Occurrence of Bacterial Leaf Spot of Tomato Caused by Pseudomonas cichorii in Taiwan
Symptom appearance of the tomato inoculated with the isolated bacteria. Two tomato cultivars (A) ‘Jiao Nu’ and (B) ‘Hong Fan’ showed dark green-dark brown water-soaking leaf spots after inoculation.
Symptom appearance of the tomato inoculated with the isolated bacteria. Two tomato cultivars (A) ‘Jiao Nu’ and (B) ‘Hong Fan’ showed dark green-dark brown water-soaking leaf spots after inoculation.

Author:Chia-Hsin Tsai*, Pao-Jen Ann, Yun-Sheng Lu, Mei-De Chen, Shu-Ling Hwang, and Yu-Hua Peng

Abstract:

    During 2010–2011 an unknown disease of tomato was found in Nantou and Hualien counties. Dark green to dark brown irregular or round spots were observed on the diseased leaves. The bacteria isolated from the infected tissues can induce tobacco hypersensitive reaction and their pathogenicity was verified by Koch’s postulates. The pathogen was further identified as Pseudomonas cichorii based on physiological and biochemical tests, Biolog identification, 16S rDNA sequence analysis, and specific primers Sf1/SfR2 following polymerase chain reaction. This is the first report of P. cichoriielicited tomato bacterial leaf spot disease in Taiwan. Among the tested agrochemicals, the mixture of streptomycin and tetracycline has the highest efficacy in inhibiting bacterial growth.

Key words:Tomato, Bacterial leaf spot, Pseudomonas cichorii, Agrochemical screening

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