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Evaluation of Real-time PCR Assay for Detection of Didymella bryoniae
Relative quantification of Didymella bryoniae by real-time PCR from various parts (root, stem, upper leaf, bud, lower leaf) of watermelon seedlings and those from soil. Healthy ctrl, healthy plant as negative control, NTC, no template control.
Relative quantification of Didymella bryoniae by real-time PCR from various parts (root, stem, upper leaf, bud, lower leaf) of watermelon seedlings and those from soil. Healthy ctrl, healthy plant as negative control, NTC, no template control.

Author:Cheng-Ping Kuan*, Yen-Hua Huang, Ching-Shan Tseng, Tsung-Chun Lin, and Hsin-Der Shih

Abstract:

    In this study, a specific and highly sensitive real-time PCR assay with TaqMan probe was developed as a tool for the diagnosis of gummy stem blight of watermelon by targeting the rDNA genes of Didymella bryoniae. In addition, the real-time PCR assay was compared to a conventional PCR method with their specificity and sensitivity on the detection of D. bryoniae in culture andin planta. The real-time PCR assay was effective for quantifying the fungal genomic DNA in the infected plants. This method was highly specific to Didymella and it was about one hundred times more sensitive than the conventional PCR method. The real-time PCR assaydeveloped isavaluable tool for detection and titer quantitation of gummy stem blight diseases on watermelon.

Key words:Gummy stem blight, Didymella bryoniae, Real-time PCR, Detection

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Journal of
Taiwan Agricultural Research
Vol. 73, No.1 (2024) ISSN: 2790-086X Vol. 73, No.1 (2024)
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