Journal of Taiwan Agricultural Research

Author:Li-Yu Tsao* and Jaw-Neng Lin

Abstract:

    Lettuce mosaic virus was isolated and identified from infected lettuce plants (Lactuca sativa var. crisp. L.) by using host reactions, electron microscopy, biological and serological assays in Taiwan. Upon artificial inoculation to 27 plant species belonging to 7 families, the virus infected lettuces (Lactuca sativa var. asparagina, L. sativa var. capitata, L. sativa var. crispa, and L. scariola var. sativa), spinach (Spinacea oleracea), pea (Pisum sativum) and tobacco (Nicotiana benthamiana) showed systemically mosaic symptoms. It developed systemically yellow lesions on Chenopodium quinoa, but only local necrotic lesions displayed on C. amaranticolor and G. glodosa. No other tested plants with symptoms were observed. The virus coat protein was purified by SDS-PAGE, from which the antiserum against LMV was produced. The titer of the antiserum against homogenous antigen in the crude sap was 5,000, tested by the indirect ELISA. The dilution end point of the crude sap detected by the antiserum was 1/640. Ten commercial lettuce seed lots were tested with the antiserum and the incidence of LMV was determined in range of 0–28%. Seven lots of lettuce seedlings collected from markets. Among them, 0–13.3% of the seedlings were positive to the LMV when detected by the antiserum. By testing 113 lettuce plants randomly collected from Mailiao Township of Yunlin County, the incidence percentage of LMV was 37%.

Key words:Lactuca sativa L., Lettuce mosaic virus, Antiserum, Detection

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