Journal of Taiwan Agricultural Research

Author:Chin-Yi Tsao, Uei-Chern Chen, Tzu-Ying Wu, and Chi-Ni Hsia*

Abstract:

    In vitro studieswere conducted to establish protocol for micropropagation of Rehmannia glutinosa Libosch, an important Chinese herbal medicine. Results showed that seeds disinfection with 0.6% sodium hypochlorite for 15 min significantly reduced the contamination rate to 5% and obtained the highest germination rate to 80%. Comparing the regeneration rate of leaf and petiole explants from in vitro grown seedlings revealed that petiole explants growing on MS medium containing 0.01 mg L^-1 1-Naphthaleneacetic acid with 2.0 mg L^-1 Benzyladenine (BA) and cultivated in darkness obtained a better survival rate (62%) along with 10% shoot induction rate and 62% callus induction rate. Cultivating shoot tip and nodal stem segment of in vitro grow seedlings on the basal medium containing various sucrose concentrations (3–9%) showed that addition of 3% sucrose had the highest survival rate (100%). Furthermore, shoot tip, inter-nodal and basal nodal stem segments of seedlings were cultured on the basal medium containing 3% sucrose and various BA concentrations (0.0–1.0 mg L^-1) to evaluate their regenerative potential. The results showed that shoot tip had the highest survival rate (80–100%) and had the most adventitious shoots induced per explant when cultured on the medium containing 0.5 mg L^-1 BA. The results show that type of stem segment explants and BA concentration are key factors influence the micorpropagation of Rehmannia glutinosa Libosch.

Key words:Rehmannia glutinosa Libosch, Micropropagation, In vitro seed germination, Shoot induction

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