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Author:Cheng-Ping Kuan*, Yu-Chang Lin, Ching-Shan Tseng, Ying-Huey Cheng, and Ting-Ching Deng
Abstract:
In this study, a diagnostic assay for Cucurbit chlorotic yellows virus (CCYV) using TaqManbased real-time reverse transcription polymerase chain reaction (qRT-PCR) was developed. Specific primers were designed by targeting the coat protein gene of CCYV. The sensitivity of qRT-PCR for the detection of CCYV when compared to that of RT-PCR assay was approximately 100–1,000 times higher. The assay is highly specific for CCYV only, no cross-reaction was observed on cucurbits infected with other cucurbit viruses using this assay. The technique is sensitive and accurate, and allows rapid detection and quantitation of CCYV in both field and experimental material used for the surveillance and diagnosis of CCYV.
Key words:Cucurbit chlorotic yellows virus, Detection, Real-time RT-PCR
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Taiwan Agricultural Research
ISSN: 2790-086X
Vol. 73, No.1 (2024)
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