Journal of Taiwan Agricultural Research

Author:Yih-Juh Shiau* and Guan-Wen Chen

Abstract:

    Strawberry (Fragaria × ananassa Duch.) is one of the perennial crops with the high economic value. It belongs to the Rosaceae family and is distributed and cultivated in more than 70 countries. Therefore, the research on long-term storage of strawberry germplasm is important. The major objective of this study is to establish an optimum axillary bud cryopreservation method of non-cold hardened strawberry ‘Taoyuan No.1’. The results obtained on pretreatment of axillary buds (bud size: 1–2 mm) prior to plunge into liquid nitrogen (LN) at -196℃ were as follows: (1) The average survival rate of buds was 96.7% ± 5.8% when the buds were precultured on MS basal medium supplemented with 0.3 M sucrose for 14 d; (2) By increasing sucrose level from 0.6 M to 1.6 M in loading solution (LS) and then osmoprotected for 90 min, the survival rate of axillary buds remained 100%; (3) The axillary buds survived was 86.7% ± 4.7% when treated with plant vitrification solution 2 (PVS2) for dehydration for 60 min. This survival percentage was significantly higher compared to PVS2 dehydration treatment for 180 min (46.7% ± 4.7%) and 240 min (46.7% ± 17.0%). After storage in LN, cryotubes were rapidly rewarmed in a water bath at 38℃ for 60−90 s. The survival rates of axillary bud beads in the range of 66.7−80% were achieved after a subculture for 14 d. There were no significant differences in survival percentages of revived axillary buds among the storage duration in LN. These results appear promising for a simplifying cryopreservation method of strawberry axillary buds.

Key words:Fragaria × ananassa Duch., Cryopreservation, Loading solution, Osmoprotection, Plant vitrification solution 2

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