Journal of Taiwan Agricultural Research

Author:Han-Wei Chen, Min-Tze Wu, and Uei-Chern Chen*

Abstract:

    Micropropagated banana (Musa spp. ‘Formosa’) requires discret management to acclimatize to the external environment. In this study, aluminum foil was replaced by non-woven fabric to improve ventilation efficiency of culture vessel headspace air condition. Different ventilation treatments of culture vessel were investigated, including hermetically sealed for 6 weeks (S6V0), 4 weeks sealed followed by 2 weeks ventilation (S4V2), 2 weeks sealed followed by 4 weeks ventilation (S2V4), and ventilation for 6 weeks (S0V6). No ethylene accumulation was detected in S4V2, S2V4, and S0V6 treatments; however, high ethylene level (203.3 nL L^-1) under airtight vessels of S6V0 was noticed and promoted adventitious root formation on banana plantlets. The stomatal index in S2V4 and S0V6 seedlings was significantly higher than plantlets subjected to the airtight condition, and stomatal densities increased with increasing duration of ventilation. Permanently widely-open stomata in detached leaves were noticed in the S6V0 and S4V2 seedling, the stomatal closure rate were 6.67% and 26.64% respectively. Since the survival of tissue cultured banana plantlets may depend largely on the prevention of water loss, factors that enhance the stomatal functionality of in vitro seedling are important. Ventilation treatments in this study showed no ethylene accumulation, in which seedling performed normal stomatal response and better vigor index value than airtight cultivation vessels. The results would be useful for industrial production of in vitro banana seedlings.

Key words:Ventilation, Banana tissue-culture, Stomatal index

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