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Increasing Drought Tolerance of Aroma Rice ‘CNY911303’ Using Marker-Assisted Selection
The different scales of rice morphology in response to drought treatment. Rice was treated with drought during 3-leaf seeding and scaled as 0: none treatment (A)、 1: Some leaves are rolled or discolored (B)、 3: Some leaves tips are drying (C)、 5: About 1/2 leaves of the plant are drying
The different scales of rice morphology in response to drought treatment. Rice was treated with drought during 3-leaf seeding and scaled as 0: none treatment (A)、 1: Some leaves are rolled or discolored (B)、 3: Some leaves tips are drying (C)、 5: About 1/2 leaves of the plant are drying

Author:Chieh-Wei Kuo, Keung-Hung Lin, Yong-Pei Wu, Yann-Rong Lin, Jen-Hao Hsu, and Yu-Chia Hsu*

Abstract:

    To introgress the gene(s) conferring drought-tolerance to the Taiwanese aromatic japonica line ‘Tainung-Chianung-yu 931103’ (‘CNY911303’), the drought-tolerant variety ‘Hang-yu 15’ (‘HY15’) was used as a donor parent crossed to ‘CNY911303’. Four backcrossings were conducted and the progenies of each backcross generation BCxF1 were screened for drought tolerance by the treatment of 28% PEG-6000 solution and consequently selected by molecular markers to recover the recurrent parent’s genome. The percentages of recurrent parent recovery within selected individuals were 74.1, 87.7, 95.9, and 96.6% in BC2F1, BC3F1, BC4F1, and BC4F2 respectively. The ‘HY15’, ‘CNY911303’ and four BC4F4 drought-tolerant lines were further evaluated for drought tolerance under the treatment of 28% PEG-6000 at three-leaf, five-leaf, and seven-leaf stages. The four BC4F4 lines exhibited higher tolerance to drought stress than the recurrent parent, implying that the drought-tolerant characteristic of ‘HY15’ was successfully transferred to ‘CNY911303’ using marker assisted selection (MAS). In addition, 4 drought-tolerant BC4F4 lines were evaluated for grain yields. Among them, ‘DT73’, ‘DT85’, and ‘DT86’ have significant better yield than ‘CNY911303’, and ‘DT73’ produced more than 5,000 kg per hectare, indicating that these three drought-tolerant lines can be grown in fields underwater saving cultivation system. These drought-tolerant lines will be registered as new aromatic rice varieties for water-saving rice production in Taiwan in the future.

Key words:Marker-Assisted Selection (MAS), Drought tolerance, Rice

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Journal of
Taiwan Agricultural Research
Vol. 73, No.2 (2024) ISSN: 2790-086X Vol. 73, No.2 (2024)
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