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Simultaneous Quantification of Seven Coumarins in Angelica dahurica (Bai Zhi) Using Ultra Performance Liquid Chromatography and Its Whitening Efficiency Assessment
The chromatograms of ultra performance liquid chromatography (UPLC) of seven authentic coumarins. 1:cinnamic acid, 2: cinnamic acid, 3: umbelliferon, 4: porsalen, 5: bergapten, 6: xanthotoxin, and 7: 8-hydroxybergapten.
The chromatograms of ultra performance liquid chromatography (UPLC) of seven authentic coumarins. 1:cinnamic acid, 2: cinnamic acid, 3: umbelliferon, 4: porsalen, 5: bergapten, 6: xanthotoxin, and 7: 8-hydroxybergapten.

Author:Shu-Chin Lo

Abstract:

    An improved ultra performance liquid chromatography method was developed to simultaneously quantify seven coumarins isolated from various organs of Bai Zhi [Angelica dahurica (Fish.) BENTH. et HOOK]. An efficient separation and quantification of coumarin can be established within 12 min using ethylene bridged hybrid (BEH) C18 column and a gradient elution system with a acetonitrile concentrations varying from 15 to 95% (v/v) in acidic water (pH 3.0) elution. The furanocoumarins such as psoralen, xanthotoxin, bergapten, and 8-hydroxybergapten were detected in 9 month-old Bai Zhi roots, in which the content of 8-hydroxybergapten was 10-fold higher than other coumarins. Nevertheless, stems from 3 and 6 month-old Bai Zhi exhibited the highest inhibitory ability on tyrosinase activity. In the future, coumarins extracted from the stems of Bai Zhi with high inhibitory effect on tyrosinase could be further used in the cosmetic industry.

Key words:Angelica dahurica whitening efficiency, Coumarins, Ultra-performance liquid chromatography

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Journal of
Taiwan Agricultural Research
Vol. 73, No.1 (2024) ISSN: 2790-086X Vol. 73, No.1 (2024)
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