Journal of Taiwan Agricultural Research

Author:Sian-Yi Ciou, Yi-Zhen Zhao, Ya-Han Chang, Chin-Hsiung Hung*, and Li-Ru Chen

Abstract:

    The experiments were conducted to study the effects of plant growth regulators on the growth of mycelia and the formation of primordia and fruiting body of Pleurotus ostreatus cultured in liquid medium. The mycelia of P. ostreatus were inoculated in culture medium containing 1 mg L^-1 or 6 mg L^-1 α-naphthaleneacetic acid (NAA), 2,4-Dichlorophenoxyacetic acid (2,4-D), or 0.2 mg L^-1 or 1.5 mg L^-1 6-Benzylaminopurine (BA) at 25℃ without shaking. The results showed that the growth of mycelia in liquid medium containing 1.5 mg L^-1 BA or 1, 6 mg L^-1 2,4-D attained 7.5–7.8 cm after inoculation for 21 d. However, the treatment of 6 mg L^-1 NAA inhibited the growth of mycelia. Twenty-one days after inoculation, treatment of 1 mg L^-1 2,4-D significantly induced the formation of primordia and fruiting bodies (P < 0.05) with an avg. of 235.5 and 5.2, respectively. The application of 1.5 mg L^-1 BA significantly reduced the number of primordia to the lowest (58.4 in avg.), and 6 mg L^-1 2,4-D treatment inhibited the formation of fruit bodies (2.6 in avg.). Study indicated that application of 1 mg L^-1 2,4-D in liquid medium can significantly enhance the growth of mycelia and the formation of primordia and fruit body of P. ostreatus. The scale-up experiments based on this study will be conducted for optimizing the commercial production of P. ostreatus in the future.

Key words:Fruit body formation, Liquid culture, Plant growth regulator, Pleurotus ostreatus

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