Journal of Taiwan Agricultural Research

Author:Uei-Chern Chen, Tzu-Ying Wu, Jhin-Yi Tsao, and Chi-Ni Hsia*

Abstract:

    An improved method for in vitro bulblet growth and rooting of Hippeastrum hybridum was established in this study. In vitro bulblets of Hippeastrum hybridum ‘Tainung No. 1’, ‘Red Lion’, and ‘Blossom Peacock’ were used as materials to conduct the liquid-solid or solid-solid two-stage culture, and additional paclobutrazol was added into culture medium to enhance growth and rooting of in vitro Amaryllis bulblets. In vitro bulbs (5 mm diameter) of ‘Tainung No. 1’ were cultured in liquid or solid MS medium containing 1 mg L^-1 BA and 0.1 mg L^-1 NAA for 8 wk of culture before transferring to a solid MS medium with the same components for another 8 wk of culture. The results showed that liquid-solid culture was superior to solid-solid culture on fresh weight of bulblets, leaf number, leaf length, and root number. In vitro bulblets of ‘Red Lion’ and ‘Blossom Peacock’ showed severe browning or death after 8 wk of culture on the control medium. Therefore, it is suggested that the subculture interval should not exceed 8 wks. The root number of ‘Red Lion’ bulblets by 50–75 mg L^-1 PBZ treatment was found significantly higher than that of control. No significant difference among PBZ treatments was found. However, root numbers of ‘Blossom Peacock’ were reduced treated with 25–75 mg L^-1 PBZ concentrations. Leaf with shorter and wider shape was observed in 75 mg L^-1 PBZ treatment. However, a lower concentration of 5 mg L^-1 PBZ was found beneficially to bulb diameter, root number and root length.

Key words:Amaryllis, Liquid-solid culture, Plant growth retardant, PP333

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