Journal of Taiwan Agricultural Research

Author:Jyh-Nong Tsai, Pao-Jen Ann*, Chu-Ping Lin, and Hui-Ling Tsai

Abstract:

    From 2006 to 2012, a total of 32 isolates of Ganoderma sp. were isolated from betel palm in seven orchards located at Kaohsiung City, Neipu and Wanluan of Pingtung County, and Caotun of Nantou County in Taiwan. In the field, Ganoderma could infect root and stem base of betel palm, causing root and stem base brown rot and necrosis, and eventual death. Basidiocarps emerging from stem base of the diseased betel palm were stipitate, or sessile. Upper surface of pileus was brown to dark, red-brown, and laccate. Pore surface was pale yellow to grey and pores were circular to oval. Basidiocarps could be produced when cultured on sawdust medium, but were smaller in size than those grown in the field. Inoculation of purified Ganoderma isolates on betel palm showed brown rot symptom, and the same Ganoderma was reisolated, confirming the pathogenicity. Based on morphological characteristics, including fruiting body, pore surface and basidiospores, and result of ITS region (ITS1-5.8S rDNA-ITS2) sequence similarity searching, done via Basic Local Alignment Search Tool (BLAST) provided from National Center for Biotechnology Information (NCBI), Ganoderma infecting betel palm in Taiwan was identified as Ganoderma boninense.

Key words:Betel palm, Ganoderma boninense

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