Journal of Taiwan Agricultural Research

Author:Uei-Chern Chen, Chin-Yi Tsao, Tzu-Ying Wu, and Chi-Ni Hsia*

Abstract:

    Efficient protocols for in vitro multiplication of Zingiber officinale ‘Guang Dong’ and ‘Chu’ were established in this study. Newly germinated rhizome buds (about 1 cm) were treated with 75% alcohol for 30 s, followed by 0.6% NaOCl for 15 min, before cultured on a B5 basal medium containing 0.4 mg L^-1 6-benzyladeninepurine (BA) for 12 wk to establish in vitro culture. Proliferation of 4.8–6.0 plantlets per explant with 1.8–2.6 cm in height were obtained by using 5 mm rhizome explants culturing on a B5 solid medium containing 0.5–2.0 mg L^-1 BA and 0.1 mg L^-1 α-naphthalene acetic acid (NAA) for ‘Guang Dong’ and ‘Chu’. Good rooting was found for all plantlets derived from all treatments. Although more plantlets were obtained in the 4.0 mg L^-1 BA treatment, shorter plantlets were observed. On the contrary, higher plantlets with less numbers were found from the control. Proliferation of 7.6–7.8 and 7.1–7.3 plantlets per explant were obtained for ‘Guang Dong’ and ‘Chu’, respectively, by using 5 mm rhizome explants culturing on the liquid medium containing 0.5–2.0 mg L^-1 BA for 8 wk. However, plantlets with unfolded leaves of 1.2–1.3 cm in height were observed for both varieties. Uncut explants of plantlets with unfolded leaves, green shoot-buds, and yellowish shootbuds derived from the liquid culture were subcultured onto the fresh solid B5 medium containing 0.5 mg L^-1 BA and 0.1 mg L^-1 NAA for another 8 wk. Proliferation of 11.2–13.0 plantlets per explant with 2.8–3.1 cm in height and 13.3–14.9 plantlets per explant with 4.6–5.2 cm in height were obtained for ‘Guang Dong’ and ‘Chu’, respectively. This result indicated that using liquid-solid two-stage culture could be beneficial on in vitro multiplication of Z. officinale.

Key words:Zingiber officinale, Liquid-solid culture, 6-benzylaminopurine, Mass propagation

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