Journal of Taiwan Agricultural Research

Author:Shu-Hui Yang* and Chien-Ya Hung

Abstract:

    The combination of a photosensitizer and a specific light source to generate reactive oxygen species for the inhibition of microbial growth is a developing antibacterial technique. In this study, the chlorophyll of spinach was degraded to prepare photosensitizer chlorophyll derivatives. The results of high performance liquid chromatography (HPLC) analysis showed that the main component was pheophytin or pheophorbide. The photosensitivity could be enhanced and resulted in the decrease of survival rate when pheophytin or pheophorbide was added to Sandos inbred mice thioguanine/ ouabain-resistant (STO) cells, Propionibacterium granulosum and Staphylococcus sp. (BCRC10783), with light-emitting diode (LED) blue light at illuminance of 5.6 J m^-2 60 min^-1, 2.8 J m^-2 10 min^-1 and 2.8 J m^-2 30 min^-1, respectively. The results indicate that chlorophyll derivatives have the photosensitivity property and can be applied as an antibacterial photosensitizer.

Key words:Chlorophyll derivative, Photosensitizer, Antibacterial, LED blue light

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