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Degradation Test of Hypochlorous Acid and Its Application in the Disinfection of Vitis thunbergii Sieb. & Zucc. Stem Node Explants

Fig. 2.　(A) The growth appearance of <i>Vitis thunbergii</i> Sieb. & Zucc. stem node segment after being disinfected with 200 mg L<sup>-1</sup> hypochlorous acid (HOCl) and inoculated into the culture medium for 7 d, and (B) after 21 d of cultivation, new leaves and buds have developed from some stem node explant. (Scale bar = 0.5 cm)

Fig. 2.　(A) The growth appearance of Vitis thunbergii Sieb. & Zucc. stem node segment after being disinfected with 200 mg L^-1 hypochlorous acid (HOCl) and inoculated into the culture medium for 7 d, and (B) after 21 d of cultivation, new leaves and buds have developed from some stem node explant. (Scale bar = 0.5 cm)

Author:Yih-Juh Shiau*, Ming-Te Lu, and Tsung-Chun Lin

Abstract:

Hypochlorous acid (HOCl) possesses potent oxidative capabilities and can disrupt the permeability of microbial cell membranes, thereby achieving sterilization. The main purpose of this study was to determine the impact of different light illuminations or high-temperature autoclaved sterilization on the degradation of HOCl. Furthermore, the study explored the effects of external disinfection treatments with different concentrations of HOCl on reducing the contamination rate of Vitis thunbergii Sieb. & Zucc stem node explants. The results showed that the stability of the solution containing 198.3 ± 3.4 mg L^-1 of HOCl could be maintained for more than 5 d if it was placed in brown glass bottled indoors at room temperature in the shade. Minimal alteration in the HOCl content was observed after autoclaving. When 0.6% (w/v) sodium hypochlorite (NaOCl) solution was used for external disinfection of Vitis thunbergii Sieb. & Zucc stem nodes for 20 min, the explant contamination rate was 82.2 ± 1.6% (Control), which was significantly higher than the treatments using 150 and 200 mg L^-1 HOCl solutions (61.1 ± 5.7% & 57.8 ± 4.2%). After a 21-day culture period, HOCl demonstrated no adverse effects on the normal growth of stem nodes. Thus, replacing NaOCl with HOCl for external disinfection of explants appears to be a viable alternative.

Key words:Autoclaving, Light illumination, Microbial contamination, Tissue culture

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Taiwan Agricultural Research

ISSN: 2790-086X Vol. 73, No.1 (2024)

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