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Optimization for the Anthocyanin Extraction from Purple Sweet Potato Roots, Using Response Surface Methodology
3-D mesh plot (A) and contour plot (B) of the effect of HCI concentration and extraction time on absorbance of extracts at 50℃
3-D mesh plot (A) and contour plot (B) of the effect of HCI concentration and extraction time on absorbance of extracts at 50℃

Author:Che-Lun Huang, Wayne-Chang Liao, Chin-Feng Chan, and Yung-Chang Lai*

Abstract:

    Anthocyanins were successfully extracted from ‘TNG 73’ purple sweet potato roots by using acidic-ethanol extraction.  Three extraction parameters including HCl concentration, extraction time, and temperature were evaluated.  Response surface methodology (RSM), an effective statistical technique was applied to establish a regression model.  The coefficients of the regression model were calculated and used to predict the extraction performance and determine the optimal operating conditions.  In conclusion, the optimal conditions for the ‘TNG 73’ anthocyanin extraction were determined to be 12% HCl (1.5 N HCl in EtOH), 45 min, and 50℃.

Key words:Anthocyanins, Purple sweet potato, Extraction, Response surface methodology

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