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In Vitro Plantlets Acclimation and Tanshinones Analysis of Salvia Miltiorrhiza
In vitro shoots rooting and ex vitro plantlets acclimation of Salvia miltiorrhiza plantlets in field. Rooting of in vitro shoots of Salvia miltiorrhiza after culture on half-strength MS medium containing 1 or 0.5 mg/L IBA for 6 wks (A), Plantlets, on half-strength MS medium containing 0.5 mg/L IBA, containers capped with 2 layers of aluminum foil for 2 wks (AF2) then replaced by 3 layers of dispense paper for 4 wks (DP4) (total 6 wks incubation )(B), then acclimatized for 4 wks (C), Bar = 2cm and 12 wks (D), Bar = 5 cm. Micropropagated plants of Salvia militiorhiza growing at the Insitute farm for six months (E), Bar = 10 cm, and the aenial and root parts of harvested plants (F), Bar = 5 cm.
In vitro shoots rooting and ex vitro plantlets acclimation of Salvia miltiorrhiza plantlets in field. Rooting of in vitro shoots of Salvia miltiorrhiza after culture on half-strength MS medium containing 1 or 0.5 mg/L IBA for 6 wks (A), Plantlets, on half-strength MS medium containing 0.5 mg/L IBA, containers capped with 2 layers of aluminum foil for 2 wks (AF2) then replaced by 3 layers of dispense paper for 4 wks (DP4) (total 6 wks incubation )(B), then acclimatized for 4 wks (C), Bar = 2cm and 12 wks (D), Bar = 5 cm. Micropropagated plants of Salvia militiorhiza growing at the Insitute farm for six months (E), Bar = 10 cm, and the aenial and root parts of harvested plants (F), Bar = 5 cm.

Author:Uei-Chern Chen, Chin-Yi Tsao and Chi-Ni Hsia*

Abstract:

    An efficient micropropagation protocol for producing high quality plantlets that are easy to acclimatize in field was developed for Salvia miltiorrhiza. In vitro grown shoots induced rooting (90%) after culturing on half-strength Murashige and Skoog’s basal medium (1/2MS) supplemented with 0.5 mg L-1 indole-3-butyric acid (IBA). Culture vessels were capped with aluminum foil for 2 weeks, and thereafter replaced with 3 layers of dispense paper for 4 weeks (AF2+DP4) were better than controls capped with aluminum foil for 6 weeks (AF6). Rooted shoots were transplanted into plastic pot containing a mixture of BioMix: vermiculite: perlite (1: 1: 1 ratio) (v/v). For acclimation, these pots were covered with transparent plastic bags and kept in a growth chamber under 14 h light at 22℃ and 10 h dark at 18℃ for 4 weeks. At 4 weeks of observation, these conditions resulted in the highest survival rate of 80.1% compared to AF6 treatment (27.8%). To compare, tanshinones contents (tanshinone-I, tanshinone-IIA and cryptotanshinone), the bioactive compounds in Salvia miltiorrhiza were estimated in the aerial parts and roots between in vitro micropropagated plants and market crude drug of S. miltiorrhiza by high performance liquid chromatography (HPLC). The quantity of tanshinones in red roots of in vitro micropropagated plants was measured as 1.85 mg g-1 dw about 1.5-fold higher than market crude drug.

Key words:Danshen, Medicinal plant, Micropropagation, High performance liquid hromatography (HPLC), Secondary metabolite

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Journal of
Taiwan Agricultural Research
Vol. 73, No.1 (2024) ISSN: 2790-086X Vol. 73, No.1 (2024)
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