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Effects of Plant Growth Regulator and Light Treatment on Callus Proliferation and In Vitro Mini-tuber Formation of Corydalis yanhusuo
In vitro callus proiferation and mini-tuber formation of Corydalis yanhusuo. Callus proliferated on MS basal salts medium containing 1 mg/L NAA, 1mg/L TDZ, 3% sucrose, and 1% difco Bacto-agar in darkness for 4 weeks of culture, Bar = 15 mm(A), and mini-tubers, Bar = 5 mm(B), somatic embryo (SE) and somatic seeding formatted on MS basal salts medium containing 0.5 mg/L IBA 3% surcorse, and 1% Difco Bacto-agar under light condition for 8 weeks of culture, Bar = 2.5 mm (C, D), Somatic seedling grew-up on half-strength MS basal medium without phytohormone for 4 weeks of culture, Bar = 5mm(E).
In vitro callus proiferation and mini-tuber formation of Corydalis yanhusuo. Callus proliferated on MS basal salts medium containing 1 mg/L NAA, 1mg/L TDZ, 3% sucrose, and 1% difco Bacto-agar in darkness for 4 weeks of culture, Bar = 15 mm(A), and mini-tubers, Bar = 5 mm(B), somatic embryo (SE) and somatic seeding formatted on MS basal salts medium containing 0.5 mg/L IBA 3% surcorse, and 1% Difco Bacto-agar under light condition for 8 weeks of culture, Bar = 2.5 mm (C, D), Somatic seedling grew-up on half-strength MS basal medium without phytohormone for 4 weeks of culture, Bar = 5mm(E).

Author:Uei-Chern Chen, Yih-Juh Shiau, Hsin-Sheng Tsay and Chi-Ni Hsia*

Abstract:

    A two-stage micropropagation system for Corydalis yanhusuo W. T. Wang, an important traditional Chinese medicinal plant, through callus proliferation followed by light treatment for tuber formation has been established in this study. The best callus proliferation was obtained under darkness on a Murashige and Skoog’s (MS) basal medium supplemented with 1 mg/L α-naphthaleneacetic acid (NAA) in combination with 1 mg/L thidiazuron (TDZ). An 11-fold increasing of callus fresh weight was recorded after 4 weeks of culture. Although callus growth was inhibited in the same growth regulator treatments while cultured under the illumination, it was found that tuber formation increased along with some somatic embryogenesis. Among all auxin treatments, phytohormone-free, 0.5-2 mg/L indole-3-acetic acid (IAA) or indole-3-butyric acid (IBA) had the best mini-tuber formation under light culturing condition with tubers range from 23 to 28 mini-tubers per 0.2 g callus. Conclusion from our results, a two-step production protocol for in vitro micropropagation of C. yanhusuo was suggested. Callus would first proliferate on a MS basal medium containing NAA and TDZ in darkness, after subculturing into the MS basal medium only or containing lower concentration of IBA (0.5 mg/L) under light condition, a good number of mini-tubers would form from callus. Mini-tuber micropropagation with many advantages superior to seeds or in vitro seedling proliferation system, and by providing good quality tuber propagules for cultivation of C. yanhusuo may be beneficial for development and utilization of medicinal resource in Taiwan.

Key words:Yan-hu-suo, Medicinal plant, Callus, Micropropagation, Thidiazuron

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Journal of
Taiwan Agricultural Research
Vol. 72, No.3 (2023) ISSN: 2790-086X Vol. 72, No.3 (2023)
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