Journal of Taiwan Agricultural Research

Author:Jen-Ren Chen, Kin-Hsing Yang, Tung-Hai Tseng, Toong-Long Jeng and Min-Tze Wu*

Abstract:

    The objectives of this work were to establish simple sequence repeat (SSR) DNA fingerprinting database and characterized SSR polymorphism in local peanut cultivars (Arachis hypogaea L.). In this investigation, 12 peanut cultivars were analyzed by using 97 SSR primer pairs. Seventy-seven SSR markers showed clear patterns and a total of 102 bands were recorded. Twenty-six SSR loci revealed polymorphism 13 of them were specific for cultivar identification. The average number of alleles per locus was 1.74, and up to 6 alleles were found at one locus. Six SSR loci in tested cultivars were sequenced for allele identification. The results indicated that different type of variation in SSR loci, including single nucleotide polymorphism (SNP), tandem repeat numbers, and non-repeat sequence locus. The results revealed that SSR markers produced a higher level of DNA polymorphism than other DNA markers in cultivated peanuts.

Key words:Cultivated peanut (Arachis hypogaea L.), Molecular marker, Simple sequence repeat(SSR).

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