Journal of Taiwan Agricultural Research

Author:Wen-Li Lee*, Yung-Shing Teng and Rong-Quey Lin

Abstract:

    The random amplified polymorphic DNA (RAPD) markers were used to identify the genetic relationship among introduced lychee cultivars in this study. Among 200 different decamer primers, 23 primers were selected; the selected primers amplified a total of 259 DNA bands. The total number of amplified bands per primer varied from 9 (UBC-199) to 26 (OPG-08), on an average 11.3 bands were induced by each primer. Jaccard’s similarity coefficients for interval measure within and between cultivars were used to produce a cluster diagram using the unweighted pair-group method (UPGMA). The results showed that eighteen samples from thirteen lychee cultivars could be divided into two major groups:one included Yu ho pao, San yue hong, Zhu yeh, Xin xing, Liu yue xue, Tang bo and Gao xiong zao sheng; the other included Sha keng xiao ho, Shang shu huai, No mi zi, Kwai wei, Kwai zi and Hei yeh. The Jaccard’s similarity coefficient of Kwai wei and Kwai zi was 1.00, implying that Kwai wei and Kwai zi may be the same cultivar. From 259 RAPD marker bands, we discovered 8 specific bands for Yu ho pao, 6 for No mi zi, 7 for Sha keng xiao ho, 6 for Gao xiong zao sheng, 1 for Shang shou huai, 4 for Xin xing, 2 for Hei yeh, 1 for of Zhu yeh and 2 for Kwai wei (Kwai zi). Those specific single bands of different cultivars will be recovered from agarose gel and cloned into TA vector system for sequencing, in order to develop new specific primer sets for the use of identifying different cultivars and hybrid posterity.

Key words:Genetic variation, Lychee, RAPD markers

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