Journal of Taiwan Agricultural Research

Author:Kung-Kuo Chiang, Ien-Chie Wen*, Wei-Yu Lee, and Ching-Yi Chang

Abstract:

    In order to have a better utilization of the longan (Dimocarpus longan Lour.) germplasm, the genetic relationships of 25 longan and litchi cultivars collected by the Taiwan Agricultural Research Institute were analyzed by using ISSR markers.  A total of 134 primers were screened for their ability to produce strongly amplified products and 15 of them were selected postively.  Out of 149 DNA bands amplified by these 15 primers, 137 of which were polymorphic (92.5%).  A dendrogram based on the UPGMA cluster analysis was constructed.  All tested 24 longan cultivars were divided into four groups, including 2 major groups of longan cultivars and 2 individual cultivar groups.  Two major groups can be subdivided into seven subgroups.  Cultivar Hong Ke Ying Jhih and Hong Ke had 91% similarity indexes, indicating the closest genetic relationship in the cultivar tested.  Nevertheless, cultivar Ching Ke having only 54% similarity indexes with other tested longan cultivars indicted the farest genetic relationship to the rest longon cultivars.  The litchi cultivar-Jhuang Yuan Hong had 44% similarity indexes with other 24 longan cultivars.

Key words: Dimocarpus longan, Genetic diversity, ISSR

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